Izvorni znanstveni članak
Allele Frequencies for 15 Short Tandem Repeat Loci in Representative Sample of Croatian Population
Petar Projic
Vedrana Škaro
Ivana Šamija
Adaleta Durmić-Pašić
Naris Pojskić
Lejla Kovačević
Narcisa Bakal
Dragan Primorac
Damir Marjanović
Sažetak
Aim To study the distribution of allele frequencies of 15 short
tandem repeat (STR) loci in a representative sample of Croatian
population.
Methods A total of 195 unrelated Caucasian individuals born in
Croatia, from 14 counties and the City of Zagreb, were sampled
for the analysis. All the tested individuals were voluntary donors.
Buccal swab was used as the DNA source. AmpFlSTR® Identifiler®
was applied to simultaneously amplify 15 STR loci. Total reaction
volume was 12.5 μL. The PCR amplification was carried out in PE
Gene Amp PCR System Thermal Cycler. Electrophoresis of the
amplification products was preformed on an ABI PRISM 3130
Genetic Analyzer. After PCR amplification and separation by
electrophoresis, raw data were compiled, analyzed, and numerical
allele designations of the profiles were obtained. Deviation from
Hardy-Weinberg equilibrium, observed and expected heterozygosity,
power of discrimination, and power of exclusion were calculated.
Bonferroni’s correction was used before each comparative
analysis.
Results We compared Croatian data with those obtained from
geographically neighboring European populations. The significant
difference (at P<0.01) in allele frequencies was recorded only between
Croatian and Slovenian populations for vWA locus. There
was no significant deviation from Hardy-Weinberg equilibrium
for all the observed loci.
Conclusion Obtained population data concurred with the expected
“STR data frame” for this part of Europe.
Ključne riječi
STR population data; Identifiler; Croatian population
Hrčak ID:
16586
URI
Datum izdavanja:
15.8.2007.
Posjeta: 1.271 *