Izvorni znanstveni članak
https://doi.org/10.1515/aiht-2016-67-2809
Genotoxic effects of the carbamate insecticide Pirimor-50® in Vicia faba root tip meristems and human lymphocyte culture after direct application and treatment with its metabolic extracts
Rafael Valencia-Quintana
orcid.org/0000-0002-3396-7694
; Laboratorio “Rafael Villalobos-Pietrini” de Toxicología Genómica y Química Ambiental, Facultad de Agrobiología, Universidad Autónoma de Tlaxcala, Universidad No. 1, Col. La Loma X. Tlaxcala, Mexico
Sandra Gómez-Arroyo
; Red Temática de Toxicología de Plaguicidas UANayarit-CONACyT, Mexico
Juana Sánchez-Alarcón
; Laboratorio “Rafael Villalobos-Pietrini” de Toxicología Genómica y Química Ambiental, Facultad de Agrobiología, Universidad Autónoma de Tlaxcala, Universidad No. 1, Col. La Loma X. Tlaxcala, Mexico
Mirta Milić
orcid.org/0000-0002-9837-7185
; Institute for Medical Research and Occupational Health, Mutagenesis Unit, Zagreb, Croatia
José Luis Gómez Olivares
; Laboratorio “Rafael Villalobos-Pietrini” de Toxicología Genómica y Química Ambiental, Facultad de Agrobiología, Universidad Autónoma de Tlaxcala, Universidad No. 1, Col. La Loma X. Tlaxcala, Mexico
Stefan M. Waliszewski
; Centro de Investigaciones Biomédicas, Universidad Veracruzana, Veracruz6, Mexico
Josefina Cortés-Eslava
; Red Temática de Toxicología de Plaguicidas UANayarit-CONACyT, Mexico
Rafael Villalobos-Pietrini
; Laboratorio de Mutagénesis, Mexico
María Elena Calderón-Segura
; Laboratorio de Genotoxicología Ambiental, Mexico
Sažetak
The aim of the study was to evaluate genotoxic effects of Pirimor-50®, a pirimicarb-based formulation (50 % active ingredient), in human lymphocyte cultures and Vicia faba root meristems. Furthermore, the objective was to examine a combined influence of insecticide treatment with mammalian microsomal S9 and vegetal S10 metabolic fractions or S10 mix metabolic transformation extracts (after Vicia faba primary roots treatment with Pirimor-50®). We used sister chromatid exchange assay-SCE and measured cell cycle progression and proliferation (proportion of M1-M3 metaphases and replication index ratio-RI). Two processes were used for plant promutagen activation: in vivo activation-Pirimor-50® was applied for 4 h to the plant and then S10 mix was added to lymphocytes; and, in vitro activation-lymphocytes were treated with Pirimor-50® and S10 or S9 for 2 h. Direct treatment induced significantly higher SCE frequencies in meristems at 0.01 mg mL-1. In lymphocytes, significantly higher SCE was at 1 mg mL-1 with decrease in RI and M1-M3 metaphase proportions at 0.5 mg mL-1 and cell division stop at 2.5 mg mL1. S10 mix lymphocyte treatment showed significantly elevated SCE values at 2-2.5 mg mL-1, with cell death at 3 mg mL-1. Lymphocyte treatment with Pirimor-50® together with S9 or S10 showed slightly elevated SCE frequency but had a significant influence on RI decrease, with lowest values in S9 treatment. Since no data are available on the genotoxicity of Pirimor-50®, this study is one of the first to evaluate and compare its direct effect in two bioassays, animal and vegetal, and also the effect of plant and animal metabolism on its genotoxic potential.
Ključne riječi
proliferation kinetics; plant and animal promutagen activation; replication index; sister chromatid exchange
Hrčak ID:
170508
URI
Datum izdavanja:
14.12.2016.
Posjeta: 2.027 *