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https://doi.org/10.1515/aiht-2016-67-2910

An alternative approach to studying the effects of ZnO nanoparticles in cultured human lymphocytes: combining electrochemistry and genotoxicity tests

Gina Branica ; Radiation Protection Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Marin Mladinić ; Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia
Dario Omanović ; Division for Marine and Environmental Research, Laboratory for Physical Chemistry of Traces, Ruđer Bošković Institute, Zagreb, Croatia
Davor Želježić ; Mutagenesis Unit, Institute for Medical Research and Occupational Health, Zagreb, Croatia


Puni tekst: engleski pdf 683 Kb

str. 277-288

preuzimanja: 543

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Sažetak

Nanoparticle use has increased radically raising concern about possible adverse effects in humans. Zinc oxide nanoparticles (ZnO NPs) are among the most common nanomaterials in consumer and medical products. Several studies indicate problems with their safe use. The aim of our study was to see at which levels ZnO NPs start to produce adverse cytogenetic effects in human lymphocytes as an early attempt toward establishing safety limits for ZnO NP exposure in humans. We assessed the genotoxic effects of low ZnO NP concentrations (1.0, 2.5, 5, and 7.5 μg mL-1) in lymphocyte cultures over 14 days of exposure. We also tested whether low and high-density lymphocytes differed in their ability to accumulate ZnO NPs in these experimental conditions. Primary DNA damage (measured with the alkaline comet assay) increased with nanoparticle concentration in unseparated and high density lymphocytes. The same happened with the fragmentation of TP53 (measured with the comet-FISH). Nanoparticle accumulation was significant only with the two highest concentrations, regardless of lymphocyte density. High-density lymphocytes had significantly more intracellular Zn2+ than light-density ones. Our results suggest that exposure to ZnO NPs in concentrations above 5 μg mL-1 increases cytogenetic damage and intracellular Zn2+ levels in lymphocytes.

Ključne riječi

comet-FISH, in vitro, primary DNA damage, TP53, voltammetry

Hrčak ID:

170509

URI

https://hrcak.srce.hr/170509

Podaci na drugim jezicima: hrvatski

Posjeta: 1.121 *