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Electrophoretic investigation of the plasma calcium transportation

V. Jovanović ; Institut za medicinska istraživanja i medicinu rada Jugoslavenske akademije znanosti i umjetnosti, Zagreb
I. Šimonović ; Institut za medicinska istraživanja i medicinu rada Jugoslavenske akademije znanosti i umjetnosti, Zagreb

Sažetak

The role of plasma proteins in the calcium transportation was studied with electrophoretic methods. The plasma was 12 hours incubated with radioactive calcium at 37°C. Labelled plasma electrophoresis was carried out in the barbiturate medium with the aid of discontinual and continual technique. The amount of plasma protein bound radioactive calcium was determined by the method of ultrafiltration. Separation of radioactive calcium in plasma, in ultrafiltrate and in the concentrated ultrafiltration residue was carried out by the discontinued electrophoresis. Electrophoresis was performed at the field intensity of 7 V/cm in the period of 2 hours so that the zone of calcium corresponding to ionized calcium was also found on the electrophoretic band. In the zone of proteins considerably less calcium was retained than it would have corresponded to the protein hound calcium obtained by the method of ultrafiltration. Similar results were obtained by the technique of continual electrophoresis. In this work a modification was also applied of the method for study of plasma protein calcium transportation. In the course of continual electrophoresis in the barbiturate medium over the separated zones of proteins has, namely, run the zone of calcium-45. At the determined condition of the study it was shown that albumins, beta and gama globulins may take part in the transportation of calcium.

Ključne riječi

Hrčak ID:

177664

URI

https://hrcak.srce.hr/177664

Datum izdavanja:

28.6.1967.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.154 *