Kratko priopćenje

https://doi.org/10.11613/BM.2017.043

Detection of let-7 miRNAs in urine supernatant as potential diagnostic approach in non-metastatic clear-cell renal cell carcinoma

Michal Fedorko ; Department of Urology, University Hospital Brno and Masaryk University Brno, Brno, Czech Republic
Jaroslav Juracek ; Masaryk University, Central European Institute of Technology, Brno, Czech Republic
Michal Stanik ; Masaryk Memorial Cancer Institute, Department of Urologic Oncology, Brno, Czech Republic
Marek Svoboda ; Masaryk Memorial Cancer Institute, Department of Comprehensive Cancer Care, Brno, Czech Republic
Alexandr Poprach ; Masaryk Memorial Cancer Institute, Department of Comprehensive Cancer Care, Brno, Czech Republic
Tomas Buchler ; Department of Oncology, Thomayer Hospital and Charles University First Faculty of Medicine, Prague, Czech Republic
Dalibor Pacik ; Department of Urology, University Hospital Brno and Masaryk University Brno, Brno, Czech Republic
Jan Dolezel ; Masaryk Memorial Cancer Institute, Department of Urologic Oncology, Brno, Czech Republic
Ondrej Slaby ; Masaryk University, Central European Institute of Technology, Brno, Czech Republic

Sažetak

IntroductionUrinary microRNAs (miRNAs) are emerging as a clinically useful tool for early and non-invasive detection of various types of cancer. The aim of this study was to evaluate whether let-7 family miRNAs differ in their urinary concentrations between renal cell carcinoma (RCC) cases and healthy controls. Materials and methodsIn the case-control study, 69 non-metastatic clear-cell RCC patients and 36 gender/age-matched healthy controls were prospectively enrolled. Total RNA was purified from cell-free supernatant of the 105 first morning urine specimens. Let-7 family miRNAs were determined in cell-free supernatant using quantitative miRNA real-time reverse-transcription PCR and absolute quantification approach. ResultsConcentrations of all let-7 miRNAs (let-7a, let-7b, let-7c, let-7d, let-7e and let-7g) were significantly higher in urine samples obtained from RCC patients compared to healthy controls (P < 0.001; P < 0.001; P = 0.005; P = 0.006; P = 0.015 and P = 0.002, respectively). Subsequent ROC analysis has shown that let-7a concentration possesses good ability to differentiate between cases and controls with area under curve being 0.8307 (sensitivity 71%, specificity 81%). ConclusionsWe have shown that let-7 miRNAs are abundant in the urine samples of patients with clear-cell RCC, and out of six let-7 family members, let-7a outperforms the others and presents promising non-invasive biomarker for the detection of RCC.

Ključne riječi

renal cell carcinoma; urine microRNAs; let-7; diagnostic biomarker

Hrčak ID:

183392

URI

https://hrcak.srce.hr/183392

Datum izdavanja:

15.6.2017.

Posjeta: 1.351 *