Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2016.990
Production and Characterization of an Alkaline Lipase from Thermophilic Anoxybacillus sp. HBB16
Z. Burcu Bakir
orcid.org/0000-0002-9241-0749
; Department of Biology, Adnan Menderes University, Aydın-09010, Turkey
K. Metin
; Department of Biology, Adnan Menderes University, Aydın-09010, Turkey
Sažetak
A thermophilic lipase-producing bacterium (Anoxybacillus sp. HBB16) was analyzed using 16S rRNA. The maximum growth rate and intracellular lipase production occurred at 50 °C and pH 6.5. Among the various nitrogen and carbon sources tested, meat extract, olive oil and olive mill wastewater (OMW) were the best sources for lipase
production. Enzyme production increased when the strain HBB16 was grown at a 180 rpm shaking speed. The maximum activity of the lipase occurred at 55 °C and pH 9.5. The presence of phenylmethylsulfonyl fluoride (PMSF), N-cyclohexyl-N′-(2-morpholinoethyl) carbodiimidemetho-p-toluenesulfonate (CMC), N-bromosuccinimide (NBS) and sodium dodecyl sulfate (SDS) inhibited enzyme activity. Bivalent metal ions caused a significant inhibition in enzyme activity, whereas univalent metal ions displayed no negative effects.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
Anoxybacillus sp.; lipase production; characterization; thermophilic; olive mill wastewater
Hrčak ID:
187141
URI
Datum izdavanja:
4.10.2017.
Posjeta: 1.658 *