Veterinarski arhiv, Vol. 87 No. 6, 2017.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.160727
Virulence gene profiles of Pasteurella multocida strains isolated from cattle and buffalo
Darioush Gharibi
; Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Mohammad R. H. Hajikolaei
; Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Masoud Ghorbanpour
; Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Seyyedeh K. Barzegar
; Graduated of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Sažetak
Pasteurella multocida is responsible for numerous economically relevant diseases in domestic animals worldwide. In cattle and buffaloes the organism is associated with hemorrhagic septicemia (HS) and bovine respiratory disease (BRD). The aim of this study was to investigate twelve virulence associated genes in 22 strains of P. multocida isolated from slaughtered cattle and buffaloes. The most frequently detected genes among bovine isolates were ptfA, nanH, exbBD-tonB and oma87; whereas hgbB and toxA genes occurred less frequently. Some of the adhesions, sialidases, iron acquisition and protectin proteins occurred at considerably (P<0.05) higher frequencies in bovine isolates. The prevalence of oma87, exbBD-tonB and hgbA genes from buffaloes was significant (P<0.05), whereas the prevalence of hgbB, ompH, pfhA and toxA genes was much lower. All tested strains of P. multocida contained the sodC gene and only 22.7% of them had sodA. By using the virulence gene profiles, 12 and 21 different gene combinations were identified among the strains isolated from cattle and buffaloes, respectively, of which Profile C1 was the most common, with all strains possessing toxA. Our results indicate the presence of virulence factors (VFs) in P. multocida strains isolated from the tested cattle and buffaloes. The occurrence of these factors in apparently healthy animals could possibly indicate early infection or a contained infection which did not lead to disease. Moreover, differences in the frequency of these factors may indicate variations in the pathogenicity of the organism.
Ključne riječi
virulence gene profile; Pasteurella multocida; buffalo; cattle
Hrčak ID:
189348
URI
Datum izdavanja:
9.11.2017.
Posjeta: 1.696 *