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https://doi.org/10.18047/poljo.23.2.5

Sterilization of different explant types in micropropagation of CAB-6p and Gisela 6 cherry rootstock

Aleksandar Stanisavljević ; Sveučilište Josipa Jurja Strossmayera u Osijeku, Poljoprivredni fakultet u Osijeku, Vladimira Preloga 1, 31000 Osijek, Hrvatska
Dejan Bošnjak ; Sveučilište Josipa Jurja Strossmayera u Osijeku, Poljoprivredni fakultet u Osijeku, Vladimira Preloga 1, 31000 Osijek, Hrvatska
Ivna Štolfa ; Sveučilište Josipa Jurja Strossmayera u Osijeku, Odjel za biologiju, Cara Hadrijana 8 / A, 31000 Osijek, Hrvatska
Rosemary Vuković ; Sveučilište Josipa Jurja Strossmayera u Osijeku, Odjel za biologiju, Cara Hadrijana 8 / A, 31000 Osijek, Hrvatska
Toni Kujundžić ; Sveučilište Josipa Jurja Strossmayera u Osijeku, Poljoprivredni fakultet u Osijeku, Vladimira Preloga 1, 31000 Osijek, Hrvatska
Mato Drenjančević ; Sveučilište Josipa Jurja Strossmayera u Osijeku, Poljoprivredni fakultet u Osijeku, Vladimira Preloga 1, 31000 Osijek, Hrvatska


Puni tekst: engleski pdf 928 Kb

str. 31-37

preuzimanja: 566

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Sažetak

Research with the objective of finding efficient (non residual) sterilants for the purpose of greater automatization in establishing aseptic in vitro tissue culture was conducted on cherry rootstocks CAB 6P and Gisela 6. Two ways of sterilization were examined (NaOCl and ozone O3) through survival rate on three types of explants; buds with primordial leaves, buds without primordial leaves and nodal non lignified segment with axillary buds. Ozone resulted with the high rate of survival (from 57 to 93%) regardless of the type of explants and the rootstock variety. By introducing a complete bud without destruction significantly resulted with the higher rate of survival (from 90 to 97%). Results indicate the potential for ozone sterilization of the starting material not only as an ostensible but the perforated (sub-cuticular transpiration) agent.

Ključne riječi

in vitro, CAB-6P, Gisela 6, sterilization, ozone

Hrčak ID:

191582

URI

https://hrcak.srce.hr/191582

Podaci na drugim jezicima: hrvatski

Posjeta: 1.328 *