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Minoxidil acts as an antiandrogen: a study of 5α-reductase zype 2 gene expression in a human keratinocyte cell line

Erkin Pekmezci ; Gözde Group Hospitals, MalatyaDermatology Department
Murat Türkoğlu ; Biota Laboratories, Istanbul

Puni tekst: engleski PDF 146 Kb

str. 271-271

preuzimanja: 4.772



Although more than three decades have passed since the first useof minoxidil in androgenetic alopecia (AGA), its mechanisms of action havestill not been comprehensively understood. 5α-reductase (5α-R) has an activerole as the predominant enzyme in both AGA and female pattern hair loss(FPHL), which are also the main therapeutic indications of topical minoxidil.But there is insufficient literature data regarding the interaction of minoxidiland the enzyme 5α-R. Herein, we studied the in vitro expression levels of 5α-Rtype 2 (5α-R2) in a minoxidil-treated human keratinocyte cell line (HaCaT) inorder to elucidate the relation of these two parameters. Cell proliferation assaywas performed by a XTT reagent (a yellow tetrazolium salt). After determinationof non-cytotoxic concentration, HaCaT cells were treated with minoxidil.Ribonucleic acid (RNA) isolations were carried out from both non-treated andtreated cell groups using a TRI reagent (an RNA, DNA, and protein isolationreagent). Gene expressions of 5α-R2 as study material and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the control were determined by realtime-quantitative polymerase chain reaction (RT-qPCR) analysis. Results wererepresented as 5α-R2 / GAPDH fold change. Minoxidil treatment resulted in a0.22 fold change for 5α-R2 (p < 0.0001). This antiandrogenic effect of minoxidil,shown by significant downregulation of 5α-R2 gene expression in HaCaT cells,may be one of its mechanisms of action in alopecia.

Ključne riječi

minoxidil, 5α-R, mechanism of action

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