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A Kinetic Study of the Inhibition of Protein Synthesis by Several Antibiotics

M. Protić ; Department of Organic Chemistry and Biochemistry »Ruaer Boskovic« Institute, Bijenicka 54, 41000 Zagreb, Croatia, Yugoslavia
Ž. Kućan ; Department of Organic Chemistry and Biochemistry »Ruaer Boskovic« Institute, Bijenicka 54, 41000 Zagreb, Croatia, Yugoslavia


Puni tekst: engleski pdf 5.520 Kb

str. 425-435

preuzimanja: 120

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Sažetak

The effects of several antibiotics on the initial reaction rates
of the polyphenylalanine and polylysine syntheses were measured
in cell-free systems from Escherichia coli MRE600, stimulated by
polyuridilic and polyadenylic acid, respectively. lVIichaelis-Menten
··type of dependence on the tRNA concentration, and a sigmoidal
(possibly cooperative) dependence on the polynucleotide concentration
was obtained. Puromycin and chloramphenicol were competitive
inhibitors with respect to tRNA, while erythromycin,
streptomycin and chlorotetracycline were non-competitive inhibitors.
On the other hand, erythromycin was apparently a competitive
inhibitor with respect to the synthetic polynucleotide, while
other above-mentioned antibiotics showed either uncompetitive,
non-competitive or mixed type of inhibition. This kinetic approach
enabled the calculation of inhibition constants for the examined
antibiotics; they ranged from 2.5 x 10-7 mol dm·3 for erythromycin
and streptomycin, to over 1 · 10·4 mol dm-a for puromycin.

Ključne riječi

Hrčak ID:

195867

URI

https://hrcak.srce.hr/195867

Posjeta: 228 *