Kratko priopćenje

https://doi.org/10.11613/BM.2018.020901

Storage stability of commonly used haematological parameters at 33 °C

Ashish Jain ; Department of Transfusion Medicine, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India
Sanchit Jain ; M.B.B.S. student, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India
Neha Singh ; Department of Pathology and Laboratory Medicine, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India
Priyanka Aswal ; Department of Pathology and Laboratory Medicine, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India
Shweta Pal ; Department of Pathology and Laboratory Medicine, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India
Sushant Kumar Meinia ; Department of Transfusion Medicine, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India
Nilotpal Chowdhury ; Department of Transfusion Medicine, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India; Department of Pathology and Laboratory Medicine, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India

Sažetak

Introduction: This study aimed to investigate the analytical bias in haematological parameters induced by storage at 33 ºC.
Materials and methods: Blood from the diversion pouch of 20 blood donors were collected in K2EDTA vials and stored at 33 ºC. Readings from
each vial were taken at 0, 4, 6, 12, 24, 48 and 72 hours after collection on the Sysmex XP-100 analyser (Sysmex Corporation, Kobe, Japan). The percent
difference from the baseline readings were calculated and subjected to a Wilcoxon signed rank test at a Holm corrected significance level of 0.05. A
median percent difference, which was statistically significant and greater than the maximum acceptable bias (taken from studies of biological variation),
was taken as evidence of unacceptable shift. If the median shift was lesser than the maximum acceptable bias, two one-sided Wilcoxon signed
rank tests for equivalence were used to determine whether the percent differences were significantly lesser than the maximum acceptable bias.
Results: Haemoglobin, red blood cell count, white blood cell count, mean corpuscular haemoglobin and lymphocyte count showed acceptable bias
after storage for at least 24 hours at 33 ºC. Haematocrit, mean corpuscular volume, mean corpuscular haemoglobin concentration, platelet count and
mean platelet volume showed unacceptable shift in less than 4 hours when stored at 33 ºC.
Conclusions: Since many haematological parameters show unacceptable bias within 4 hours of sample storage at 33 ºC, the recommended limit of
time from collection to processing should be revised for areas where high environmental temperatures are common.

Ključne riječi

pre-analytical error; erythrocyte indices; laboratory errors; platelet count; sample stability

Hrčak ID:

199000

URI

https://hrcak.srce.hr/199000

Datum izdavanja:

15.6.2018.

Posjeta: 773 *