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Evaluation of symptomatic patients with resistant discharge

Cemile Sonmez ; Public Health General Directorate, Ministry of Health, Ankara
Selma Usluca ; Public Health General Directorate, Ministry of Health, Ankara
Ismail Hakki Usluca ; Numune Training and Research Hospital, Gynecology and Obstetrics Clinic, Ankara
Irem Kalipci ; Numune Training and Research Hospital, Gynecology and Obstetrics Clinic, Ankara
Figen Sezen ; Public Health General Directorate, Ministry of Health, Ankara
Cemal Resat Atalay orcid id orcid.org/0000-0002-0148-0945 ; Numune Training and Research Hospital, Gynecology and Obstetrics Clinic, Ankara
Selcuk Kilic ; Public Health General Directorate, Ministry of Health, Ankara.University of Health Sciences, Istanbul Medical Faculty, Department of Medical Microbiology, Istanbul.


Puni tekst: engleski PDF 133 Kb

str. 1-1

preuzimanja: 230

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Sažetak

The aim of this study was to detect the presence of Chlamydia trachomatis, Neisseria (N.) gonorrhoeae, Mycoplasma (M.) hominis, M. genitalium, Ureaplasma (U.) urealyticum, and Trichomonas (T.) vaginalis in patients with resistant discharge. The study also evaluated the concordance of the diagnostic tests. Samples from 156 patients were tested by direct microscopy and culture for T. vaginalis and Mycoplasma IES for M. hominis and U. urealyticum. Multiplex Polymerase Chain Reaction (PCR) was used to determine the presence of six agents. Statistical analyses were performed using the SPSS program. Out of 156 patients, 38 had positive result for the agents tested. Of these 38 patients, 28 (73.7%) had single agent positivity and 10 (26.3%) had multiple agent positivity. The detection rate of U. urealyticum, M. hominis, N. gonorrhoeae, C. trachomatis, T. vaginalis, M. genitalium specifically was 10.3%, 9.6%, 6.4%, 3.2%, 2.6%, 0.6% respectively. N. gonorrhoeae and U. urealyticum were the most common in male patients, while M. hominis and U. urealyticum were mostly found in female patients. Different methods used for detecting T. vaginalis were compared to find that interrater reliability was perfect for culture-direct microscopy (κ:0.85; P<0.001) and also for culture-PCR (κ:0.89; P<0.001). The interrater reliability was moderate (κ:0.53; P<0.001) for PCR-Mycoplasma IES test for M. hominis and fair (κ:0.21; P<0.007) for U. urealyticum. U. urealyticum and M. hominis were among the most commonly found sexually transmitted infections (STI) agents in patients with resistant discharge. Multiple agent positivity was high and should be kept in mind in every STI case.

 

Ključne riječi

Ureaplasma urealyticum, Mycoplasma hominis, diagnostic methods

Hrčak ID:

199810

URI

https://hrcak.srce.hr/199810

Posjeta: 455 *