Izvorni znanstveni članak
https://doi.org/10.5513/JCEA01/19.2.2151
Use of MALDI-TOF mass spectrometry in rapid identification of Beauveria bassiana and Beauveria pseudobassiana
Nikola Hricakova
orcid.org/0000-0001-8363-3740
; Department of Microbiology, Faculty of Biotechn ology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovakia
Juraj Medo
; Department of Microbiology, Faculty of Biotechn ology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovakia
Lukáš Hleba
; Department of Microbiology, Faculty of Biotechn ology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovakia
Marek Barta
; Arboretum Mlyňany, Slovak Academy of Sciences, Arboretum Mlyňany SAV, Slepčany, Slovakia
Jana Makova
; Department of Microbiology, Faculty of Biotechn ology and Food Sciences, Slovak University of Agriculture in Nitra, Nitra, Slovakia
Sažetak
Species of the genus Beauveria are entomopathogenic fungi used in biological control to protect against insect pests. Identification of species is based on observation of morphological, phylogenetic and genetic differences. Currently, MALDI-TOF (Matrix Assisted Laser Desorption/Ionization-Time of Flight) mass spectrometry is often used in microbial diagnostic but identification of Beauveria species has not been tested before. The aim of this work was the possibilities of this method and to compare it with genetic analysis. Twenty strains of B. bassiana and B. pseudobassiana isolated from insect cadavers, mycoinsecticides and soil samples were used. Sequences of ITS (Internal Transcribed Spacer) region were used for genetic identification of strains. Sequences and results from protein analysis of isolates were compared with the reference strains of B. bassiana and B. pseudobassiana. Results of MALDI Biotyper softvare identification were correct for all strains. Identification was reliable with high log score (>2.1) for most of strains. Score value less than 1 was observed only in a single case - strain GHA isolated from mycoinsecticide Botanigard.The result of both analyses was also dendrogram (phylogenetic tree) which showed high degree of result consistence. MALDI-TOF identification of fungi from Beauveria genus is usable for fast and reliable identification of entomopathogenic fungi isolates acquired from environment and results can be utilized in conservation biocontrol strategies in agriculture and forest ecosystems.
Ključne riječi
Beauveria; genetic analysis; spectrum analysis of proteins
Hrčak ID:
201840
URI
Datum izdavanja:
20.6.2018.
Posjeta: 1.501 *