Croatica Chemica Acta, Vol. 91 No. 2, 2018.
Izvorni znanstveni članak
https://doi.org/10.5562/cca3349
Non-canonical Structures in Promoter Modulate Gene Expression in Escherichia coli
Sabina Božič Abram
; Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
Maja Marušič
; Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
Martina Lenarčič Živković
; Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
Jasna Brčić
; Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
Janez Plavec
orcid.org/0000-0003-1570-8602
; Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, SI-1000 Ljubljana, Slovenia
Sažetak
Herein we show how sequences that can form different non-canonical structures affect gene expression levels when inserted in the core of σ70-dependent promoter, between the −35 and −10 elements recognized by RNA polymerase, in E. coli. We note that influence on level of GFP expression varies considerably depending on introduction of non-canonical structural elements in the antisense and sense strands as well as with their propensities to form G-triplex, G-hairpin, hairpin or G-quadruplex structures. Moreover, the extent of repression of expression does not relate to the in vitro thermal stability in a simple manner. Repression is most likely caused by steric interference rather than improper distance between the −35 and −10 elements. Although properties like thermal stability and topology can be somewhat different under in vivo and in vitro conditions, our results suggest that the extent of expression suppression cannot be dependent solely on thermal stabilities of G-rich structures alone.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
DNA; non-canonical structures; topology; gene expression modulation; E. coli σ70; GFP expression
Hrčak ID:
202771
URI
Datum izdavanja:
4.6.2018.
Posjeta: 1.291 *