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https://doi.org/10.11613/BM.2018.030707

Analysis of selected T cell subsets in peripheral blood after exhaustive effort among elite soccer players

Dorota Kostrzewa-Nowak ; Department of Biological Bases of Physical Education, Faculty of Physical Education and Health Promotion, University of Szczecin, Szczecin, Poland; Centre for Human Structural and Functional Research, Faculty of Physical Education and Health Promotion, Un
Robert Nowak ; Department of Biological Bases of Physical Education, Faculty of Physical Education and Health Promotion, University of Szczecin, Szczecin, Poland; Centre for Human Structural and Functional Research, Faculty of Physical Education and Health Promotion, Un

Sažetak

Introduction: Physical exercise induces developing of naïve T lymphocyte subsets into polarised effector ones by immune system. The aim of the
study was to examine the influence of exhaustive effort on the selected Th cell subsets and inflammation markers among soccer players.
Materials and methods: Fourteen soccer players aged 18 (16-21) years old performed the progressive efficiency test on mechanical treadmill. Th
and Tc memory lymphocytes’ subsets and selected cytokine concentrations pre-exercise, post-exercise and in recovery were analysed by flow cytometry.
Results: Physical effort induced changes in Th cell percentages. Increase in recovery Treg and Th17 cell subsets’ percentages in comparison to preexercise
values were observed (10.98 (9.83-14.07) vs. 3.95 (3.15-5.53), P < 0.001 in autumn; 10.58 (7.54-12.67) vs. 4.83 (3.73-6.81), P < 0.010 in
spring for Treg and 29.21 (26.34-32.16) vs. 21.64 (18.48-25.76) in autumn; 27.15 (24.60-29.16) vs. 17.43 (15.83-19.77) in spring, both P < 0.010; for
Th17, respectively). Increases in Th1 cell percentages post-exercise (31.86 (28.72-33.72) in autumn, 25.60 (21.50-29.19) in spring, both P < 0.010) and
in recovery (34.64 (31.21-38.20) in autumn; 26.68 (25.17-28.07) in spring, both P < 0.001) compared to pre-exercise (22.70 (21.21-26.74) for autumn
and 15.64 (14.38-19.63) for spring, respectively) were found. Interestingly, no changes in Th2 cell subsets were found. Post-exercise and recovery
changes in IL-6, IL-8, TNF-α and IL-10 were also observed.
Conclusions: It seems that the given effort in the progressive test induced an anabolic effect being related not only with cytokine profile but also
with CD4+ T cells’ differentiation and peripheral distribution.

Ključne riječi

athletes; fatigue; flow cytometry; soccer; T-lymphocytes

Hrčak ID:

206659

URI

https://hrcak.srce.hr/206659

Datum izdavanja:

15.10.2018.

Posjeta: 610 *