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A Quantitative Chromatographic Method for the Determination of Leucine Aminopeptidase Activity

D. Lebez ; Department of Radiobiology, Nuclear Institute »Joief Stefan«, Ljubljana, Slovenia, Yugoslavia
M. Funtek ; Department of Radiobiology, Nuclear Institute »Joief Stefan«, Ljubljana, Slovenia, Yugoslavia


Puni tekst: engleski pdf 3.281 Kb

str. 293-295

preuzimanja: 67

citiraj


Sažetak

A micro method for the determination of leucine aminopeptidase
activity was developed. Leucinamide was us ed as substrate. The
liberated leucin e was quantitative ly determined using paper chromatography
a nd reflectanc e-densitometrical scanning of the spots.
From the spot area of leucine the enzyme activity was evaluated.
This method is useful for the determination of leucine aminopeptidase
activity in supernatants obtained after centrifugation of
homogenized organs.

Ključne riječi

Hrčak ID:

208077

URI

https://hrcak.srce.hr/208077

Posjeta: 133 *