Izvorni znanstveni članak
https://doi.org/10.2478/aiht-2018-69-3154
Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay
Dijana Topalović
orcid.org/0000-0001-6546-2626
; Department of Biology and Human Genetics, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia
Dragana Dekanski
; Biomedical Research, R&D Institute, Galenika a.d., Belgrade, Serbia
Biljana Spremo-Potparević
; Department of Biology and Human Genetics, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia
Ninoslav Djelić
; Department of Biology, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia
Vladan Bajić
; Laboratory for Radiobiology and Molecular Genetics, Institute for Nuclear Research “Vinča”, University of Belgrade, Belgrade, Serbia
Lada Živković
; Department of Biology and Human Genetics, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia
Sažetak
Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 μmol L-1 adrenaline or 50 μmol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 μmol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.
Ključne riječi
DNA repair; hydrogen peroxide; genotoxicity; quercetin; ROS
Hrčak ID:
213624
URI
Datum izdavanja:
20.12.2018.
Posjeta: 1.730 *