Sažetak sa skupa
Structural characterisation of novel exopolysaccharide biosynthesized by potential probiotic strain Lactobacillus fermentum D12
Katarina Butorac
orcid.org/0000-0002-1682-0703
; Faculty of Food Technology and Biotechnology, Laboratory for Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, University of Zagreb, Pierottijeva 6, Zagreb, Croatia
Barbara Bellich
orcid.org/0000-0002-5257-6584
; Department of Life Sciences, University of Trieste, Via Licio Giorgieri 1 Ed. C11-34127 Trieste, Italy
Martina Banić
; Faculty of Food Technology and Biotechnology, Laboratory for Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, University of Zagreb, Pierottijeva 6, Zagreb, Croatia
Andreja Leboš Pavune
orcid.org/0000-0002-4954-7133
; Faculty of Food Technology and Biotechnology, Laboratory for Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, University of Zagreb, Pierottijeva 6, Zagreb, Croatia
Jasna Novak
; Faculty of Food Technology and Biotechnology, Laboratory for Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, University of Zagreb, Pierottijeva 6, Zagreb, Croatia
Ksenija Durgo
; Faculty of Food Technology and Biotechnology, Laboratory for Biology and Microbial Genetics, University of Zagreb, Pierottijeva 6, Zagreb, Croatia
Paola Cescutti
; Department of Life Sciences, University of Trieste, Via Licio Giorgieri 1 Ed. C11-34127 Trieste, Italy
Roberto Rizzo
; Department of Life Sciences, University of Trieste, Via Licio Giorgieri 1 Ed. C11-34127 Trieste, Italy
Slaven Zjalić
orcid.org/0000-0002-5123-7687
; Department of Ecology, Agronomy and Aquaculture, University of Zadar, Trg kneza Višeslava 9, 23000 Zadar, Croatia
Jagoda Šušković
; Faculty of Food Technology and Biotechnology, Laboratory for Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, University of Zagreb, Pierottijeva 6, Zagreb, Croatia
Blaženka Kos
; Faculty of Food Technology and Biotechnology, Laboratory for Antibiotic, Enzyme, Probiotic and Starter Culture Technologies, University of Zagreb, Pierottijeva 6, Zagreb, Croatia
Sažetak
Lactobacillus fermentum D12, biosynthesize exopolysaccharides, is released in large amounts in MRS broth supplemented with glucose. High Performance Size Exclusion Chromatography (HPSEC), 1H-NMR, GC and GC-MS analysis revealed that this strain produces three different types of EPOLs; the first homopolysaccharide (HoPOL) of a molecular weight of 400 kDa and two different low molecular weight heteropolysaccharides (HePOLs) of less than 2 kDa. 2D-NMR spectroscopy analysis revealed that HoEPOL, with the highest molecular mass, is composed of repeating units of D-glucose linked by an α-1,4-glycosidic bond, where 20% of the glucose subunits is acetylated at C-3. Further chromatographic analyses and NMR experiments showed that each HePOL contained mannose, glucose and galactose in an averaged relative molar ratio of 1.78:0.87:1 and 6.38:1.6:1, respectively. Since a probiotic strain survival in rigorous gastrointestinal (GI) conditions is the first probiotic selection criterion to be met, and with respect to efficient survival of D12 strain in GIT in vitro (bacteria counts ≥106 CFU ml-1) the potential probiotic role of Lb. fermentum D12 was evaluated. Also, sensitivity to different antibiotics, minimum inhibitory concentrations (MICs), antagonistic activity and analysis of fermentation of different carbohydrates using API 50 CHL media of this potential probiotic strain was assessed.
Ključne riječi
Lactobacillus, exopolysaccharides, probiotic activity; structural characterization
Hrčak ID:
225499
URI
Datum izdavanja:
18.9.2019.
Posjeta: 600 *