Veterinarski arhiv, Vol. 89 No. 4, 2019.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.0340
CYP11B1 and PPARGC1A genes polymorphism controlling reproductive traits and estimation of breeding value of first lactation milk yield in Bos indicus (Deoni) cattle
Sukanta Basak D. N. Das
; Animal Genetics and Breeding, Indian Council of Agricultural Research-National Dairy Research Institute, Southern Regional Station, Bengaluru, India
Upendra T. Mundhe
; Animal Genetics and Breeding, Indian Council of Agricultural Research-National Dairy Research Institute, Southern Regional Station, Bengaluru, India
Sažetak
In the present study, in Deoni, the dual purpose cattle breed of Southern India, maintained in a National Dairy Research Institute herd, Southern Regional Station, Bengaluru, molecular characterization of the putative exon 1 of CYP11B1 and putative intron 9 and 3’UTR of PPARGC1A genes was carried out in 146 animals using the PCR-RFLP technique. Three restriction enzymes, namely PstI, HaeIII and NheI, were used for digestion of the amplicons of the genes, respectively. In the putative exon 1 of the CYP11B1 gene, two genotypes, VV and VA, were detected with frequencies of 0.23 and 0.77, respectively. The frequencies of allele V and A in the population were found to be 0.62 and 0.38, respectively. The allelic frequencies of C and T types were observed as 0.63 and 0.37, with frequencies of CC, TC and TT genotypes as 0.38, 0.51 and 0.11 in the putative intron 9 of the PPARGC1A gene, respectively. Three genotypes, namely AA, AC and CC were detected in 3’UTR of the PPARGC1A gene, with respective frequencies of 0.75, 0.21 and 0.04. The allelic frequencies of A and C types were 0.86 and 0.14, respectively. The locus (c.1892+19T>C) in the putative intron 9 of the PPARGC1A gene was found to be significant (P<0.05), with unadjusted data of age of first calving (AFC). Adjusted data of first lactation milk yield (FLMY) showed a significant (P<0.10) association with the loci of the CYP11B1 (p.Val30Ala) and PPARGC1A (c.3359A>C) genes. No significant (P<0.10) association was observed between the loci of the genes and breeding value of FLMY in the studied cattle population.
Ključne riječi
Deoni; first lactation milk yield; CYP11B1 gene; PPARGC1A gene
Hrčak ID:
225737
URI
Datum izdavanja:
30.9.2019.
Posjeta: 977 *