Veterinarski arhiv, Vol. 90 No. 2, 2020.
Izvorni znanstveni članak
https://doi.org/10.24099/vet.arhiv.0401
The temporal dynamics of antioxidants and lipid peroxidation in chick embryo livers after low-dose gamma irradiation
Marinko Vilić
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Jasna Aladrović
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Blanka Beer Ljubić
; Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivona Žura Žaja
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Željko Gottstein
; Department of Poultry Diseases with Clinic, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Jadranka Pejaković Hlede
; Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Marija Majer
; Division of Materials Chemistry, Ruđer Bošković Institute, Zagreb, Croatia
Selim Pašić
; Department of Physics, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Sažetak
The effects were measured of low-doses of gamma radiation within 24 hours after irradiation on the activity of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT) and levels of glutathione (GSH) and lipid peroxide concentrations (malondialdehyde, MDA) in embryonic chick liver. The chick embryos were irradiated with doses of gamma radiation of 0.05, 0.15, 0.3, 0.5 and 0.8 Gy at the dose rate of 0.0117 Gy/s on day 19 of incubation. All parameters were measured 1, 3, 6, 12 and 24 hours after irradiation by spectrophotometry, apart from the level of lipid peroxidation, which was assessed using high performance liquid chromatography (HPLC). A versatile analysis of the data was made and it consisted of: 1) a comparison of the means of antioxidant enzyme activities and GSH and MDA concentrations for each radiation dose and the time after irradiation with the corresponding control samples; 2) research into the temporal dynamics of enzyme activities and concentrations a) for each value of radiation dose and b) when the data for radiation doses were merged, i.e. regardless of the radiation doses. A significant increase in GSH- Px activities in time after irradiation was found in dose intervals from 0.05 to 0.5 Gy, which was well described by linear function. This is a highly reliable result, because it was obtained as a result of all the analyses applied. For other parameters, we did not find any dependence of activities and concentrations on time. The analysis of comparison of means of the irradiated and control samples gave statistically significant results for some dose-time pairs.
Ključne riječi
antioxidants; chick; embryo; liver; low-dose radiation; gamma irradiation
Hrčak ID:
238614
URI
Datum izdavanja:
25.5.2020.
Posjeta: 715 *