Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2018.59.327
In vitro effects of hydrogen peroxide on rat uterine contraction before and during pregnancy
Rahmah Alanazi
; of Medicine, King Saud University,Riyadh, Saudi ArabiaDepartment of Physiology, College
Mohammed Alotaibi
; of Medicine, King Saud University,Riyadh, Saudi ArabiaDepartment of Physiology, College
Laiche Djouhri
; Department of Basic Medical Sciences, College of Medicine University of Qatar, Doha, Qatar
Sažetak
Aim To assess the in vitro effect of hydrogen peroxide
(H2O2) on uterine contractions in pregnant and non-pregnant
rats.
Methods The study was performed at the Department of
Physiology, College of Medicine, King Saud University from
December 2016 to October 2017. Intact uterine samples
were obtained from non-pregnant (n = 7-8) and termpregnant
(n = 6-7) rats. Small longitudinal uterine strips
were dissected and mounted in an organ bath. Isometric
force measurements were used to assess the effect of 400,
800, and 1000 μM H2O2 on spontaneous uterine contractions
and contractions induced by oxytocin (5 nM), high
calcium (Ca+2) solution (6 mmol/L), and high potassium
chloride (KCl) solution (60 mmol/L).Results In both term-pregnant and non-pregnant uterine
strips, H2O2 elicited a biphasic response, consisting of
a transient contraction followed by a persistent decrease
in spontaneously generated contractions, contractions induced
by oxytocin, and contractions induced by high Ca+2
(all P < 0.01, compared with controls) in a concentration-dependent
manner. The effect of H2O2 was more pronounced
in non-pregnant than in pregnant rats (P < 0.05). In both
groups, H2O2 failed to relax uterine strips pre-contracted
with high-KCl solution (P > 0.05 compared with controls).
Conclusion H2O2 was shown to be a potent uterine relaxant
in pregnant and non-pregnant states. The pregnant
uterus better withstood the inhibitory effect of H2O2 than
non-pregnant uterus.
Ključne riječi
Hrčak ID:
239727
URI
Datum izdavanja:
16.12.2018.
Posjeta: 656 *