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Croatian Medical Journal, Vol. 60 No. 2, 2019.

Izvorni znanstveni članak

https://doi.org/10.3325/cmj.2019.60.78

Raman microspectroscopy: toward a better distinction and profiling of different populations of dental stem cells

Jelena Simonović ; School of Dental Medicine University of Belgrade, Belgrade, Serbia
Boško Toljić orcid id orcid.org/0000-0002-6664-2829 ; School of Dental Medicine University of Belgrade, Belgrade, Serbia
Božidar Rašković orcid id orcid.org/0000-0001-7190-5833 ; Faculty of Agriculture, Institute of Animal Sciences, University of Belgrade, Belgrade, Serbia
Vladimir Jovanović orcid id orcid.org/0000-0001-6640-4575 ; Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia
Miloš Lazarević orcid id orcid.org/0000-0003-1330-5332 ; School of Dental Medicine University of Belgrade, Belgrade, Serbia
Maja Milošević ; School of Dental Medicine University of Belgrade, Belgrade, Serbia
Nadja Nikolić ; School of Dental Medicine University of Belgrade, Belgrade, Serbia
Radmila Panajotović ; Institute of Physics, University of Belgrade, Belgrade, Serbia
Jelena Milašin orcid id orcid.org/0000-0002-6225-7210 ; School of Dental Medicine University of Belgrade, Belgrade, Serbia

Puni tekst: engleski pdf 1.331 Kb

str. 78-86

preuzimanja: 285

citiraj

Sažetak

Aim To characterize stem cells originating from different
dental tissues (apical papilla [SCAP], dental follicle [DFSC],
and pulp [DPSC]) and test the capacity of Raman microspectroscopy
to distinguish between the three dental
stem cell types.
Methods SCAP, DFSC, and DPSC cultures were generated
from three immature wisdom teeth originating from three
patients. Cell stemness was confirmed by inducing neuro-,
osteo-, chondro-, and adipo-differentiaton and by mesenchymal
marker expression analysis by flow-cytometry and
real-time polymerase chain reaction. Cellular components
were then evaluated by Raman microspectroscopy. Results We found differences between SCAP, DFSC, and
DPSC Raman spectra. The ratio between proteins and nucleic
acids (748/770), a parameter for discriminating more
differentiated from less differentiated cells, showed significant
differences between the three cell types. All cells also
displayed a fingerprint region in the 600-700 cm-1 range,
and characteristic lipid peaks at positions 1440 cm-1 and
1650 cm-1.
Conclusion Although different dental stem cells exhibited
similar Raman spectra, the method enabled us to make
subtle distinction between them.

Ključne riječi

Hrčak ID:

239734

URI

https://hrcak.srce.hr/239734

Datum izdavanja:

15.4.2019.

Posjeta: 837 *