Izvorni znanstveni članak

https://doi.org/10.2478/acph-2021-0023

Screening of six medicinal plant species for antileishmanial activity

FABIANA BORGES PADILHA FERREIRA orcid.org/0000-0001-8853-3626 ; Posgraduate Program in Health Sciences, Maringá State University, Maringá – Paraná State, Brazil
MÁRCIA REGINA PEREIRA CABRAL ; Posgraduate Program in Pharmaceutical Sciences, Maringá State University, Maringá – Paraná State, Brazil
MARIA HELENA SARRAGIOTTO ; Postgraduate Program in Chemistry, Maringá State University, Maringá – Paraná, Brazil
CARLA MARIA MARIANO FERNANDEZ ; Posgraduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense UNIPAR, Umuarama – Paraná State, Brazil
ZILDA CRISTIANI GAZIM ; Posgraduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense UNIPAR, Umuarama – Paraná State, Brazil
RANULFO PIAU JUNIOR ; Posgraduate Program in Animal Science of the University of Paranaense, Universidade Paranaense UNIPAR, Umuarama – Paraná State, Brazil
IZABEL GALHARDO DEMARCHI ; Federal University of Santa Catarina, Florianópolis – Santa Catarina State, Brazil
THAÍS GOMES VERZIGNASSI SILVEIRA ; Posgraduate Program in Health Sciences, Maringá State University, Maringá – Paraná State, Brazil; Department of Clinical Analysis and Biomedicine, Maringá State University, Maringá – Paraná State, Brazil
MARIA VALDRINEZ CAMPANA LONARDONI ; Posgraduate Program in Health Sciences, Maringá State University, Maringá – Paraná State, Brazil; Department of Clinical Analysis and Biomedicine, Maringá State University, Maringá – Paraná State, Brazil

Sažetak

This study is aimed to investigate the in vitro antileishmanial activity of ethanolic, aqueous or dichloromethane extracts of leaves, flowers, fruits or roots, of six medicinal plant species, namely, Nectandra megapotamica, Brunfelsia uniflora, Myrcianthes pungens, Anona muricata, Hymenaea stigonocarpa and Piper corcovandesis. After isolation and analysis of chemical components by ultra-high performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS), the extracts were also tested for toxicity in J774.A1 macrophages and human erythrocytes. Phenolic acids, flavonoids, acetogenins, alkaloids and lignans were identified in these extracts. Grow inhibition of promastigotes forms of Leishmania amazonensis and Leishmania braziliensis and the cytotoxicity in J774.A1 macrophages were estimated by the XTT method. The most promising results for L. amazonensis and L. braziliensis were shown by the ethanolic extract of the fruits of Hymenaea stigonocarpa and dichloromethane extract of the roots of Piper corcovadensis, with IC50 of 160 and 150 µg mL–1, resp. Ethanolic extracts of A. muricata (leaf), B. uniflora (flower and leaf), M. pungens (fruit and leaf), N. megapotamica (leaf), and aqueous extract of H. stigonocarpa (fruit) showed IC50 > 170 µg mL–1 for L. amazonensis and > 200 µg mL–1 for L. braziliensis. The extracts exhibited low cytotoxicity towards J774.A1 macrophages with CC50 > 1000 µg mL–1 and hemolytic activity from 0 to 46.1 %.

Ključne riječi

Piper corcovadensis; Hymenaea stigonocarpa; antileishmanial; Leishmania amazonensis; Leishmania braziliensis; cytotoxicity; hemolysis

Hrčak ID:

242231

URI

https://hrcak.srce.hr/242231

Datum izdavanja:

30.9.2021.

Posjeta: 733 *