Izvorni znanstveni članak
https://doi.org/10.2478/aiht-2020-71-3267
Microtubular structure impairment after GSM-modulated RF radiation exposure
Ana Marija Marjanović Čermak
; Institute for Medical Research and Occupational Health, Radiation Dosimetry and Radiobiology Unit, Zagreb, Croatia
Krunoslav Ilić
; Institute for Medical Research and Occupational Health, Radiation Dosimetry and Radiobiology Unit, Zagreb, Croatia
Ivan Pavičić
; Institute for Medical Research and Occupational Health, Radiation Dosimetry and Radiobiology Unit, Zagreb, Croatia
Sažetak
The objective of the study was to investigate whether low-level 915 MHz GSM-modulated radiofrequency (RF) radiation impairs microtubular structure and affects normal cell growth. V79 cells were exposed to a GSM-modulated field in a Gigahertz Transversal Electromagnetic Mode cell (GTEM cell) for 1, 2, and 3 h. Signal generator combined with power and chip modulator generated the electromagnetic field (EMF). The electric field strength was adjusted to 10, 20, and 30 V/m, and the average specific absorption rate (SAR) was calculated to be 0.23, 0.8, and 1.6 W/kg. The structure of microtubule proteins was assessed by indirect immunocytochemistry, and cell growth was determined based on cell counts taken every day over six post-exposure days. Three-hour radiation exposure significantly altered microtubule structure regardless of the electric field strength. Moreover, on the third post-exposure day, three-hour radiation significantly reduced cell growth, regardless of field strength. The same was observed with two-hour exposure at 20 and 30 V/m. In conclusion, 915 MHz GSM-modulated RF radiation affects microtubular proteins in a time-dependent manner, which, in turn, affects cell proliferation. Our future research will focus on microtubule structure throughout the cell cycle and RF radiation effects on mitotic spindle.
Ključne riječi
cell growth; cytoskeleton; in vitro; 915 MHz; mobile phone radiation
Hrčak ID:
243870
URI
Datum izdavanja:
22.9.2020.
Posjeta: 1.554 *