Izvorni znanstveni članak

https://doi.org/10.5562/cca3673

Chemical Fingerprinting, Total Phenolics and Antioxidant Activity of Some Iris Taxa

Ivan Duka orcid.org/0000-0001-7414-7695 ; University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Botany, Schrottova 39, 10000 Zagreb, Croatia
Željan Maleš orcid.org/0000-0003-1034-2525 ; University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Pharmaceutical Botany, Schrottova 39, 10000 Zagreb, Croatia
Mirza Bojić orcid.org/0000-0002-8381-9577 ; University of Zagreb Faculty of Pharmacy and Biochemistry, Department of Medicinal Chemistry, A. Kovačića 1, 10000 Zagreb, Croatia
Dario Hruševar orcid.org/0000-0002-8776-9685 ; University of Zagreb Faculty of Science, Department of Biology, Rooseveltov trg 6, 10000 Zagreb, Croatia
Božena Mitić orcid.org/0000-0002-0589-4248 ; University of Zagreb Faculty of Science, Department of Biology, Rooseveltov trg 6, 10000 Zagreb, Croatia

Sažetak

Some Iris taxa covered in this study were used or are still being used as remedies in European folk medicine. The most common indication for the decoction of Iris rhizomes is sore throat and productive cough, especially in case of the taxon I. domestica (L.) Goldblatt & Mabb., the well-known remedy in traditional Chinese medicine. The aim of this study is to chemically characterize rhizomes of seven wild and medicinal Iris taxa and to assess the antioxidant activity of the rhizome extracts. The HPTLC chromatograms and densitometric spectrum scan of the spots were used for untargeted metabolic profiling and tentative metabolite class identification of Iris taxa. Total number of found spots is 41, three major spots are found in all taxa, and 22 spots are found to be taxon specific. Two xanthones, three isoflavonoid glycosides, 18 isoflavonoid aglycons (isoflavonoids), and five acetophenones/benzophenones are tentatively identified. The taxon I. pallida Lam. contains largest amount of phenolic substances (28.5 ± 2.0 mg GAE / g dry plant material) and was the most effective ABTS scavenger (27.5 ± 2.4 mg TROLOXE / g dry plant material). The taxon I. illyrica Tomm. ex Vis. was the most effective in both DPPH and FRAP assays, 10.1 ± 1.5 and 17.3 ± 0.1 mg TROLOXE / g dry plant material, respectively. The most effective taxa in the metal chelating assay were I. croatica Horvat & M.D.Horvat (6.1 ± 0.1 mg EDTAE / g dry plant material) and I. germanica var. florentina (L.) Dykes (5.8 ± 0.1 mg EDTAE / g dry plant material). European Iris taxa have a relatively similar and partly shared chemical profile with Asian taxon I. domestica, which might be a sign of equivalence in the medicinal value.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Ključne riječi

genus Iris; isoflavonoids; xanthones; free radicals; FRAP; metal chelation

Hrčak ID:

244308

URI

https://hrcak.srce.hr/244308

Datum izdavanja:

24.4.2020.

Posjeta: 2.002 *