Kratko priopćenje

https://doi.org/10.24099/vet.arhiv.0930

The influence of the duration of in vitro maturation and incubation with an activating agent on the hatching capacity of bovine parthenotes - short communication

Ernesto Orozco-Lucero ; Department of Veterinary Sciences, Biomedical Sciences Institute, Autonomous University of Ciudad Juarez, Ciudad Juarez, Chihuahua, Mexico
Bianca V. Orozco-Galindo ; Department of Veterinary Sciences, Biomedical Sciences Institute, Autonomous University of Ciudad Juarez, Ciudad Juarez, Chihuahua, Mexico
Juan A. Quintero-Elisea ; Department of Veterinary Sciences, Biomedical Sciences Institute, Autonomous University of Ciudad Juarez, Ciudad Juarez, Chihuahua, Mexico
Mateo F. Itza-Ortiz ; Department of Veterinary Sciences, Biomedical Sciences Institute, Autonomous University of Ciudad Juarez, Ciudad Juarez, Chihuahua, Mexico
Marbella Chavez-Solano ; Department of Chemical and Biological Sciences, Biomedical Sciences Institute, Autonomous University of Ciudad Juarez, Ciudad Juarez, Chihuahua, Mexico
José M. Carrera-Chávez ; Department of Veterinary Sciences, Biomedical Sciences Institute, Autonomous University of Ciudad Juarez, Ciudad Juarez, Chihuahua, Mexico
Armando Varela-Ramirez ; Border Biomedical Research Center, Department of Biological Sciences, University of Texas at El Paso, El Paso, TX, USA
Andrés Quezada-Casasola ; Department of Veterinary Sciences, Biomedical Sciences Institute, Autonomous University of Ciudad Juarez, Ciudad Juarez, Chihuahua, Mexico

Sažetak

The period of both in vitro maturation (IVM) and incubation with oocyte activators affects the blastocyst yield following parthenogenetic activation (PA). Nevertheless, it is still unknown how these conditions impact the expansion and hatching rates of bovine parthenogenetic blastocysts. The objective of this study was to assess the influence of the duration of IVM and exposure to the activating agent, 6-dimethylaminopurine (6-DMAP), on a number of developmental parameters in bovine parthenotes, including: Cleavage, blastocyst formation, expansion, and hatching. Slaughterhouse oocytes were subjected to different periods of IVM. Subsequently, eggs were first parthenogenetically activated for five minutes with ionomycin and then incubated for distinct lengths of time with a second activator, 6-DMAP. The treatments were: a) Control: 22 h IVM/4 h 6-DMAP; b) 22 h IVM/5 h 6-DMAP; c) 24 h IVM/4 h 6-DMAP; and d) 24 h IVM/5 h 6-DMAP. Developmental stages were evaluated at day 4 and day 8 of in vitro culture (IVC). No differences were detected in most developmental parameters. However, the duration of IVM and incubation with 6-DMAP significantly affected (P<0.05) hatching capacity considering the number of blastocysts (Hatch./Blast.). Also, this same variable was higher (P<0.05) in group b) 22 h IVM/5 h 6-DMAP (45.89 ± 12.59%), as compared to c) 24 h IVM/4 h 6-DMAP (6.67 ± 6.67%). In conclusion, the length of IVM and incubation with 6-DMAP influenced parthenogenetic development, where 22 h IVM/5 h 6-DMAP was the condition producing the highest Hatch./Blast. rate in bovine parthenotes.

Ključne riječi

in vitro maturation; 6-dimethylaminopurine; culture length; parthenogenesis; bovine

Hrčak ID:

245825

URI

https://hrcak.srce.hr/245825

Datum izdavanja:

10.11.2020.

Podaci na drugim jezicima: hrvatski

Posjeta: 846 *