Skoči na glavni sadržaj

Izvorni znanstveni članak

https://doi.org/10.5671/ca.44.3.2

Influence of Caffeine on Crystallization and Amelioration of Oxidative Stress on in vitro Model of Urolithiasis

Vjera Ninčević ; Department of Pharmacology, Faculty of Medicine, Josip Juraj Strossmayer University, Osijek, Croatia
Tomislav Kizivat ; Department of Pharmacology and Biochemistry, Faculty of Dental Medicine and Health, Josip Juraj Strossmayer University, Osijek, Croatia
Tea Omanović Kolarić ; Department of Pharmacology, Faculty of Medicine, Josip Juraj Strossmayer University, Osijek, Croatia
Lucija Kuna ; Department of Pharmacology and Biochemistry, Faculty of Dental Medicine and Health, Josip Juraj Strossmayer University, Osijek, Croatia
Anita Cindrić ; Department of Pharmacology, Faculty of Medicine, Josip Juraj Strossmayer University, Osijek, Croatia
Anamarija Banovac ; Department of Pharmacology, Faculty of Medicine, Josip Juraj Strossmayer University, Osijek, Croatia
Ines Bilić Ćurčić ; Department of Pharmacology, Faculty of Medicine, Josip Juraj Strossmayer University, Osijek, Croatia
Sandra Tucak-Zorić ; Department of Mineral Metabolism, Faculty of Medicine, Josip Juraj Strosmayer University, Osijek, Croatia
Martina Smolić ; Department of Pharmacology, Faculty of Medicine, Josip Juraj Strossmayer University, Osijek, Croatia


Puni tekst: engleski pdf 401 Kb

str. 121-125

preuzimanja: 281

citiraj


Sažetak

Urolithiasis is a disease characterized by formation of solid crystals within the urinary tract. Kidney stone formation is still not clear but it is mostly composed of calcium oxalate which can produce free radicals that are toxic to renal tubular cells. Oxidative stress is an important contributory mechanism in cell damage and is associated with a number of disorders. Several studies have shown antioxidative effects of caffeine, proposing its possible role in stopping the formation of calcium oxalate stones in urinary tract. Hence, the aim of this study was to evaluate the toxic effects of calcium oxalate monohydrate crystals (COM) on renal epithelial cell line; Madin-Darby canine kidney cells subtype I (MDCK I) and Epithelial-like pig kidney cell line (LLC-PK1), and to determine possible inhibition of COM that caused oxidative stress by antioxidant treatment with caffeine in different concentrations in a cell culture model of urolithiasis.

Ključne riječi

urolithiasis, oxidative stress, MDCK cells, LLC-PK1 cells, calcium oxalate monohydrate, caffeine

Hrčak ID:

247630

URI

https://hrcak.srce.hr/247630

Posjeta: 696 *