Veterinarska stanica, Vol. 52 No. 2, 2021.
Pregledni rad
https://doi.org/10.46419/vs.52.2.11
Sedatives in veterinary medicine and their determination
Ada Puntarić
; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Hrvatska
Đurđica Božić Luburić
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Ivana Varenina
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Nina Bilandžić
orcid.org/0000-0002-0009-5367
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Marina Krpan
; Hrvatski veterinarski institut, Zagreb, Hrvatska
Mirjana Hruškar
; Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, Hrvatska
Sažetak
Sedatives, as veterinary medicinal products used on food-producing animals just before slaughter, pose a potential risk to consumer health due to the increased possibility of the presence of their residues in products of animal origin. For public health protection purposes, the regular control of food products, using adequate and reliable analytical methods to detect possible deviations from the prescribed values, is of high importance. The lag of sedative residues in the body of treated animals depends on many factors, such as the type of sedative used, type of animal receiving the sedative, initial dose, method and place of administration, and animal’s overall health, etc. It is essential to establish the target organ or tissue for their determination. Based on the literature data, the kidney is the most commonly examined target organ, and gives a true picture of the amount of residue present. Analytical methods used to determine sedative residues
can be screening (immunoassays - ELISA) or confirmatory (chromatography methods such as gas, thin layer, liquid chromatography/ liquid chromatography coupled with mass spectrometry). Nevertheless, the standard for their determination is liquid chromatography coupled with mass spectrometry. This method is the most reliable for determining residues of various contaminants in biological samples, not only sedatives. Nowadays, ultra high performance liquid chromatography (UHPLC) is increasingly used, thanks to its higher sensitivity, precision and resolution compared to conventional liquid chromatography. Therefore, the UHPLC-MS/MS method with its high sensitivity and selectivity outperforms other available methods. This paper describes some of the most commonly used sedatives in food-producing animals, and ways to determine their residues.
Ključne riječi
monitoring; residues; sedatives; UHPLC-MS/MS; veterinary medicinal products
Hrčak ID:
251389
URI
Datum izdavanja:
1.12.2020.
Posjeta: 2.395 *