Skoči na glavni sadržaj

Periodicum biologorum, Vol. 121-122 No. 3-4, 2020.

Pregledni rad

https://doi.org/10.18054/pb.v121-122i3-4.10818

Use of bacterial Rho helicase to gain new insights into the targeting mechanism of nuclear RNAs by the exosome-associated exoribonuclease Rrp6 and its cofactors in yeast

Igor Stuparevic orcid id orcid.org/0000-0002-9878-5007 ; University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory of Biochemistry
Kévin Moreau orcid id orcid.org/0000-0002-3686-0737 ; Centre de Biophysique Moléculaire UPR4301 du CNRS, Rue Charles Sadron, Orléans, France.
A. Rachid Rahmouni orcid id orcid.org/0000-0002-2519-1849 ; Centre de Biophysique Moléculaire UPR4301 du CNRS, Rue Charles Sadron, Orléans, France

Puni tekst: engleski pdf 814 Kb

str. 147-153

preuzimanja: 372

citiraj

Sažetak

In eukaryotic cells, aberrant mRNPs with processing and packaging defects are targeted co-transcriptionally by a surveillance system that triggers their nuclear retention and ultimately the degradation of their mRNA component by the 3’-5’ activity of the exosome-associated exoribonuclease Rrp6 along with its associated factors Rrp47 and Mpp6. This mRNP quality control process is stimulated by the NNS complex (Nrd1-Nab3-Sen1), which otherwise mediates termination, processing and decay of ncRNAs and the process involves additionally the exosome co-activator TRAMP complex (Trf4-Air2-Mtr4). Here, we describe a genome-wide approach to visualize the dynamic recruitment of these quality control components over the yeast chromosomes upon global perturbation of mRNP biogenesis by the RNA-dependent helicase/translocase activity of the bacterial Rho factor.


The method provides valuable information on how the surveillance system coordinates with the transcription machinery in order to detect faulty events during perturbation of mRNP biogenesis. In addition, our overview shows that the assembly of the quality control components for the affected mRNA genes takes place at the expense of their commitment to be recruited to ncRNAs genomic features, which in turn leads to termination and processing defects of ncRNAs.

Ključne riječi

Hrčak ID:

254656

URI

https://hrcak.srce.hr/254656

Datum izdavanja:

30.12.2020.

Posjeta: 740 *