Izvorni znanstveni članak
https://doi.org/10.5513/JCEA01/22.2.2904
Chemically defined caseinate extenders and their effect on the cooled semen collected from stallions with long sexual rest
Jana VOKROUHLÍKOVÁ
; Department of Animal Husbandry Sciences, Faculty of Agriculture, University of South Bohemia, Studentská 13, 370 05 České Budějovice, Czech Republic
Veronika ČOUDKOVÁ
orcid.org/0000-0001-7705-4195
; Department of Animal Husbandry Sciences, Faculty of Agriculture, University of South Bohemia, Studentská 13, 370 05 České Budějovice, Czech Republic
Jiří ŠICHTAŘ
; Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Praha 6-Suchdol, Czech Republic
Aneta VRBOVÁ
orcid.org/0000-0002-8977-842X
; Department of Animal Husbandry Sciences, Faculty of Agriculture, University of South Bohemia, Studentská 13, 370 05 České Budějovice, Czech Republic
Alena BÚDOVÁ
; Department of Animal Husbandry Sciences, Faculty of Agriculture, University of South Bohemia, Studentská 13, 370 05 České Budějovice, Czech Republic
Miroslav MARŠÁLEK
; Czech Hipological Society, Na zlaté stoce 690/3, 370 05 České Budějovice
Jitka RUTKAYOVÁ
; Department of Animal Husbandry Sciences, Faculty of Agriculture, University of South Bohemia, Studentská 13, 370 05 České Budějovice, Czech Republic
Sažetak
The ability of three milk-based extenders to preserve the motility and viability of cooled shipped semen produced by stallions after a long sexual rest was evaluated. In total, 21 ejaculates from 11 stallions were collected after 5–6 months of sexual inactivity and diluted with Kenney, INRA 96, and EquiPlus. Seven parameters were evaluated – spermatozoa viability (VIT, %), total motility (MOT, %), progressive motility (PMOT, %), number of progressive spermatozoa per ml (prog M/ml, million/ml), average path velocity (VAP, μm/s), curvilinear velocity (VCL, μm/s) and straight-line velocity of spermatozoa (VSP, μm/s). These parameters were determined by CASA 2 hours after semen processing and then in 24, 48 and 72-hour intervals of storage at 5 °C. The effect of storage time was highly significant (P<0,001) for all evaluated parameters. The effect of the extender was significant (P<0.05) for VIT, MOT, PMOT, VCL, VSL and VAP. The ejaculates extended with INRA 96 reached significantly (P<0.01) higher values for all evaluated parameters compared to Kenney extender. The INRA 96 extender significantly increased the values of PMOT, VCL and VAP compared to EquiPlus (P<0.05). In all observed extenders, MOT and VIT decreased linearly whereas PMOT decreased exponentially. The fastest decrease of PMOT was observed within 24 hours of storage. The results obtained from using INRA 96
showed that quality of cooled semen for stallions collected after a long period of sexual inactivity can be sufficiently maintained during at least 24 hours of storage.
Ključne riječi
cooled semen; ejaculate; extender; sperm motility; stallion
Hrčak ID:
259540
URI
Datum izdavanja:
29.6.2021.
Posjeta: 621 *