Original scientific paper
https://doi.org/10.33602/mebm.3.2.4
miR-7 AND miR-34a SEQUENCE CLONING AND EXPRESSION IN A1235 GLIOBLASTOMA CELL LINE
Dora Kolic
orcid.org/0000-0002-3498-5616
; Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Luka Horvat
; Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Maja Setinc
; Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Mariastefania Antica
; Division of Molecular Biology, Rudjer Boskovic Institute, Zagreb, Croatia
Maja Matulic
orcid.org/0000-0002-3498-5616
; Department of Molecular Biology, Faculty of Science, University of Zagreb, Zagreb, Croatia
Abstract
miRNAs are small non-coding RNAs which have an important role in signalling circuits regulating different cell processes. miR-7 and miR-34a are known as tumour suppressors, and both of them can interfere with cell proliferation, differentiation, apoptosis and migration. We constructed plasmids containing pri-miRNA sequences for these two miRNAs and introduced them into the A1235 glioblastoma cell line. Clones containing increased expression of processed miR-7 and miR-34a were obtained. The proliferation and sensitivity to alkylation agent of transfected cells were similar to those of control cells. Our results indicate that an increase in miR-7 and miR34 expression alone in A1235 glioblastoma cells is not sufficient to change their proliferation or sensitivity to the influence of alkylating agents.
Keywords
miR-7; miR-34a; expression; glioblastoma cell line
Hrčak ID:
261476
URI
Publication date:
18.12.2020.
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