Izvorni znanstveni članak

https://doi.org/10.46419/vs.53.4.2

Study of antibiotics and symbiotic effects on sperm quality using the CASA system

Ahmed El Bachir Bouchicha ; Institute of Sciences and technology, Tissemsilt University, Laboratory of Biotechnologies related to animal reproduction (LBRA), Institute of Veterinary Sciences, Saad Dahleb University, Blida, Algeria
Ammar Kalem ; Laboratory of Biotechnologies related to animal reproduction (LBRA), Institute of Veterinary Sciences, Saad Dahleb University, Blida, Algeria
Nora Mimoune orcid.org/0000-0002-0900-3908 ; Laboratory of Biorechnologies related to animal reproduction (LBRA), Institute of Veterinary Sciences, Saad Dahleb University, Blida, Algeria, National High School of Veterinary Medicine, Animal Health and Productions Laboratory, El-Alia, Algiers, Algeria
Sohaib Djouadi ; Faculty of Nature & Life Sciences, Farm Animal Reproduction Laboratory, University of Tiaret, Algeria
Djamel Khelef ; National High School of Veterinary Medicine, Animal Health and Productions Laboratory, El-Alia, Algiers, Algeria
Rachid Kaidi ; Laboratory of Biorechnologies related to animal reproduction (LBRA), Institute of Veterinary Sciences, Saad Dahleb University, Blida, Algeria

Sažetak

The objective of the current work was to study in vitro sperm quality after antibiotics and symbiotic administration and to evaluate treatment administered before insemination with the aim of reducing artificial insemination failures in goats. This experimental study was carried out at the Animal Reproduction Biotechnology Laboratory (Blida, Algeria). Semen analysis was performed using the Computer-assisted sperm analysis system. In the first experimental approach, we used the antibiotics most commonly administered in the veterinary field for the treatment of subclinical endometritis. A total of eight antibiotics were studied. Each antibiotic tested was co-incubated with frozen goat semen brought from the Centre for Artificial Insemination and Genetic Improvement. For the second experimental approach, we incubated semen with a symbiotic (Symbiovéba). Finally, we selected two antibiotics among those used, namely colistin and cotrimoxazole, and these were co-incubated with the symbiotic and the semen, to examine possible combinations of antibiotics with symbiotics in the treatment and prevention of uterine infections (broad spectrum synergistic activity). Antibiotics have been shown to have a detrimental effect on the sperm cell, by decreasing sperm motility. The average value calculated on all antibiotics was 18% (as opposed to initial motility of 78% in the control group), with an alteration of the linear speed that would have a negative impact on fertilization. On the other hand, symbiotics had a beneficial effect on spermatozoa motility and vitality. The combination of the symbiotic and colistin proved to be very promising. In conclusion, the use of symbiotics in the treatment of subclinical endometriosis at the time of goat insemination is beneficial, and requires greater attention in future research.

Ključne riječi

sperm; antibiotic; symbiotic; CASA; semen; subclinical endometritis

Hrčak ID:

261605

URI

https://hrcak.srce.hr/261605

Datum izdavanja:

23.12.2021.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.616 *