Izvorni znanstveni članak
https://doi.org/10.2478/aiht-2021-72-3541
The role of pumpkin pulp extract carotenoids against mycotoxin damage in the blood brain barrier in vitro
Manuel Alonso-Garrido
; University of València Faculty of Pharmacy, Laboratory of Food Chemistry and Toxicology, Burjassot, Spain
Noelia Pallarés
; University of València Faculty of Pharmacy, Laboratory of Food Chemistry and Toxicology, Burjassot, Spain
Guillermina Font
; University of València Faculty of Pharmacy, Laboratory of Food Chemistry and Toxicology, Burjassot, Spain
Paola Tedeschi
; University of Ferrara, Department of Chemistry and Pharmaceutical Sciences, Ferrara, Italy
Lara Manyes
orcid.org/0000-0001-5777-6107
; University of València Faculty of Pharmacy, Laboratory of Food Chemistry and Toxicology, Burjassot, Spain
Manuel Lozano
orcid.org/0000-0003-2046-5959
; University of València Faculty of Pharmacy, Laboratory of Food Chemistry and Toxicology, Burjassot; Jaume I University and University of València Foundation for the Promotion of Health and Biomedical Research in the Valencian Region (FISABIO), Epidemiology and Environmental Health Joint Research Unit, València, Spain
Sažetak
Some mycotoxins such as beauvericin (BEA), ochratoxin A (OTA), and zearalenone (ZEA) can cross the blood brain barrier, which is why we tested the anti-inflammatory action of a pumpkin carotenoid extract (from the pulp) against these mycotoxins and their combinations (OTA+ZEA and OTA+ZEA+BEA) on a blood brain barrier model with co-cultured ECV304 and C6 cells using an untargeted metabolomic approach. The cells were added with mycotoxins at a concentration of 100 nmol/L per mycotoxin and pumpkin carotenoid extract at 500 nmol/L. For control we used only vehicle solvent (cell control) or vehicle solvent with pumpkin extract (extract control). After two hours of exposure, samples were analysed with HPLC-ESI-QTOF-MS. Metabolites were identified against the Metlin database. The proinflammatory arachidonic acid metabolite eoxin (14,15-LTE4) showed lower abundance in ZEA and BEA+OTA+ZEA-treated cultures that also received the pumpkin extract than in cultures that were not treated with the extract. Another marker of inflammation, prostaglandin D2-glycerol ester, was only found in cultures treated with OTA+ZEA and BEA+OTA+ZEA but not in the ones that were also treated with the pumpkin extract. Furthermore, the concentration of the pumpkin extract metabolite dihydromorelloflavone significantly decreased in the presence of mycotoxins. In conclusion, the pumpkin extract showed protective activity against cellular inflammation triggered by mycotoxins thanks to the properties pertinent to flavonoids contained in the pulp.
Ključne riječi
beauvericin; ECV304; metabolomics; ochratoxin A; zearalenone
Hrčak ID:
262381
URI
Datum izdavanja:
17.9.2021.
Posjeta: 980 *