Veterinarska stanica, Vol. 53 No. 3, 2022.
Izvorni znanstveni članak
https://doi.org/10.46419/vs.53.3.9
Influence of immunomodulators of viral or bacterial origin and vaccine against Aujeszky’s disease on the proportion of peripheral blood B cells in growing pigs
Irena Žarković
; Croatian Veterinary Institute, Zagreb, Croatia
Miroslav Andrišić
; Croatian Veterinary Institute, Zagreb, Croatia
Ksenija Šandor
; Croatian Veterinary Institute, Zagreb, Croatia
Anja Vujnović
; Croatian Veterinary Institute, Zagreb, Croatia
Eleonora Perak Junaković
; Croatian Veterinary Institute, Zagreb, Croatia
Branka Šeol Martinec
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Marcela Šperanda
; Faculty of Agrobiotechnical Sciences, Osijek, Croatia
Hrvoje Valpotić
; Faculty of Veterinary Medicine University of Zagreb, Croatia
Andreja Jungić
; Croatian Veterinary Institute, Zagreb, Croatia
Miroslav Benić
; Croatian Veterinary Institute, Zagreb, Croatia
Luka Cvetnić
; Croatian Veterinary Institute, Zagreb, Croatia
Svjetlana Terzić
; Croatian Veterinary Institute, Zagreb, Croatia
Sažetak
The consequences of infection by Suid herpesvirus type 1 (SuHV-1) that causes Aujeszky’s disease (AD) are well studied, however, the effects of immunomodulators (IMs) of microbial origin (viral and bacterial) when administered solely or in combination with the attenuated SuHV-1 vaccine are less known. The effects of parenteral administration of IMs, inactivated Parapoxvirus ovis (P. ovis) or a combination of inactivated Propionibacterium granulosum (P. granulosum) and detoxified Escherichia coli lipopolysaccharide (LPS) and attenuated SuHV-1, strain Bartha, on the proportion of peripheral blood CD3- CD21+ B cells were analysed in 30 crossbred, 3-month old pigs using flow cytometry (FCM). Specific antibodies for gE and gB of SuHV- 1 were detected using the enzyme-linked immunosorbent assay (ELISA). Data were compared among six experimental groups: (1) pigs that separately received the vaccine, (2) IM of bacterial origin, (3) IM of viral origin, (4) simultaneous administration of the vaccine and bacterial IM, (5) simultaneous administration of the vaccine and viral IM, and (6) the control group of untreated pigs. Comparison of B cell proportions and the detection of specific antibodies in blood samples of vaccinated pigs on Day 11 of the experiment showed a transient decrease in B cell contents, though this could not be assumed to be related since the control group showed a decrease in B cell proportion on the same day. The results showed that the use of IMs alone or in combination with the attenuated SuHV-1 vaccine did not have a significant impact on the proportion of peripheral blood B cells in growing pigs.
Ključne riječi
B cells; pigs; flow cytometry; immunomodulators; Aujeszky disease
Hrčak ID:
263036
URI
Datum izdavanja:
31.10.2021.
Posjeta: 716 *