Pregledni rad

Introduction to next generation sequencing technologies

Iva Štimac
Franjo Martinković ; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska

Sažetak

Genome sequencing is a significant step in determining the correlation between genotype and phenotype traits. Sequencing technologies are important in many fields in the life and biomedical sciences. The era of sequencing development is divided into four generations. First generation sequencing includes sequencing by synthesis (Sanger sequencing) and sequencing by cleavage (Maxam-Gilbert sequencing). Sanger sequencing enabled the complete sequencing of various genomes, including human, and provided the foundation for the development of second, third and fourth-generation technologies, collectively known as “new-generation sequencing,” that overcame certain limitations of Sanger method. But despite many advantages in terms of speed, cost and parallelism, the accuracy and read length of Sanger sequencing still surpasses them and limits their use mainly to resequencing procedure. Consequently, there is a constant need to develop improved real time sequencing technologies. Following the bibliographic sources, the data given in this paper are a brief historical overview of the development of next generation sequencing technologies (NGS).

Ključne riječi

Next generation sequencing; Second-generation sequencers; Third-generation sequencers; Fourth-generation sequencers.

Hrčak ID:

263646

URI

https://hrcak.srce.hr/263646

Datum izdavanja:

12.9.2021.

Podaci na drugim jezicima: hrvatski

Posjeta: 2.814 *