Original scientific paper

https://doi.org/10.46419/vs.53.4.4

ELISA and Fourier-transform infrared spectroscopy: HER2 gene expression in the blood serum of canines with a mammary tumour

Alejandro Clavijo-Maldonado ; Grupo de Investigación GEBIOME, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Calle 65 No. 26-10 - A.A. 275, Manizales, Colombia, Grupo de Propiedades Ópticas de Materiales, Departamento de Física y Química, Universidad Nacional de Colombia Sede Manizales, Manizales, Colombia
Enio Ferreira ; Departamento de Patologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 486 31270-901, Belo Horizonte, Minas Gerais, Brasil
Jorge E. Pérez-Cárdenas ; Departamento de Ciencias Básicas, Facultad de Ciencias para la Salud, Universidad de Caldas, Calle 65 No. 26-10 - A.A. 275, Manizales, Colombia
Carlos Vargas- Hernández ; Grupo de Propiedades Ópticas de Materiales, Departamento de Física y Química, Universidad Nacional de Colombia Sede Manizales, Manizales, Colombia
Fredy A, Rivera-Páez ; Grupo de Investigación GEBIOME, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Calle 65 No. 26-10 - A.A. 275, Manizales, Colombia

Abstract

ELISA and FTIR assay techniques were used to identify HER2 gene expression in the blood serum of female dogs and to characterise the biochemical composition. ELISA tests assess the stage of primary tumour development and evolution, while FTIR allows for a complete characterisation of biomolecules associated with the tumoral process. Blood serum samples from 30 female dogs were analysed. Concentrations of the HER2/neu protein were detected using ELISA kits specific for canine and human detection. Infrared spectroscopy (IR) was conducted in absorbance mode at a frequency range of 400–4000 cm-1 and a resolution of 4 cm-1 over 50 scans. The ELISA cut-off for HER2 protein concentration in blood serum was determined using the receiver operating characteristic (ROC) curve and by estimating the area under the curve (AUC) at a 95% confidence interval (CI=95%). The ROC curves in the canine and human ELISA tests were 0.75 and 0.45, respectively. The representative IR spectra for HER2 gene expression corresponded to lipids (1161 cm-1, 1452 cm-1, 2851 cm-1). This study contributes to the knowledge of HER2 through the identification of biochemical features associated with the changes in the HER2/neu+ and HER2/neu- states.

Keywords

ErbB2; infrared spectroscopy; immunoassay; oncoprotein; blood

Hrčak ID:

264505

URI

https://hrcak.srce.hr/264505

Publication date:

23.12.2021.

Article data in other languages: croatian

Visits: 712 *