Veterinarski arhiv, Vol. 77 No. 5, 2007.
Izvorni znanstveni članak
Cytochemistry of sheep bone marrow cells
Salah Abdul Latif Al Izzi
; IDEXX Laboratories, 1345 Denison, Markham, Ontario, Canada
Mahasen Matug Gawas
; Department of Preventive Medicine, Faculty of Veterinary Medicine, Al Fateh University, Tripoli, Libya
Khalid Mohammed Belhaj
; Department of Physiology, Faculty of Veterinary Medicine, Al Fateh University, Tripoli, Libya
Sažetak
The present study was conducted on bone marrow samples obtained from 15 clinically normal Libyan Barbary sheep. Haemopoietic cells, including those of myelocytic series, erythrocytic series, megakaryocytic series, lymphocytes, plasma cells, monocytes and mitotic cells were identified on the basis of their morphological characteristics in May-Grünwald-Giemsa stained bone marrow smears. Cytochemical reactions of bone marrow cells revealed that all granulocytes, except myeloblasts and basophils, were peroxidase positive. Monocytes
were peroxidase negative, but very few of them showed weak activity. Cells of the erythrocytic, megakaryocytic and lymphocytic series were peroxidase negative. Granulocytic series cells were Sudan black B positive, except myeloblasts. Some monocytes were positive with a few scattered black granules, while others were negative. Cells of erythrocytic, megakaryocytic and lymphocytic series were Sudan black B negative. Granulocytes at all stages of development reacted positively to periodic acid-Shiff, except myeloblasts. Monocytes were either completely negative or positive. Some lymphocytes showed a few fine or even coarse periodic acid-Shiff positive granules in the cytoplasm. Megakaryocytes were periodic acid-Shiff positive, while all cells of the erythrocytic series were periodic acid-Shiff negative. There was agreement in the mean values of the percentages of the three cytochemical stains positive cells and May-Grünwald-Giemsa stained bone marrow granulocytes.
Ključne riječi
sheep; bone marrow; cytochemistry; peroxidase; Sudan black B; periodic acid-Shiff
Hrčak ID:
24760
URI
Datum izdavanja:
19.10.2007.
Posjeta: 1.701 *