Veterinarski arhiv, Vol. 77 No. 4, 2007.
Izvorni znanstveni članak
Application of nested-PCR for detection of foot-and-mouth disease viral sequences in tonsil of slaughtered cattle with clinically normal appearance in Iran
Aliasghar Bahari
; Junior School of Veterinary Medicine, Bu-Ali Sina University, Hamadan, Iran
Taghi Taghipour-Bazargani
; Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Otfried Marquardt
; Federal Research Center for Virus Diseases of Animals, Tűbingen, Germany
Seyed Ali Ghorashi
; National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
Saied Bokaie
; Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Sažetak
Persistent or inapparent infection, the so-called carrier state, is an important feature of foot-and-mouth disease (FMD) in ruminants. This may occur in non-vaccinated as well as in vaccinated ruminants following exposure to infectious FMD virus. Although the amount of infective virus that can be recovered from carriers is small, the virus can be present in some ruminants for months and in cattle for years. FMD is enzootic in Iran. The present study was carried out for determination of FMD viral genome in clinically normal cattle slaughtered
at Zyaran Abattoir. A total of 133 tonsil tissue samples was collected and total RNA was extracted from each sample individually. RT-PCR and subsequently nested-PCR were carried out on each sample using FMDV specific primers from the 3D-3A region. A 222 bp DNA fragment was amplified from positive samples. Of the 133 tonsil tissue samples, 46 were found positive. Results indicated that the frequency of FMD carriers among tested animals is 34.59%. Statistical analysis did not show any significant differences (P>0.05) between positive samples in relation to sex, age and breed. The high frequency of carriers could be due to extensive FMDV circulation among susceptible animals. This finding suggests that, at least at present, full vaccination coverage is required for domestic ruminants in Iran to increase their resistance to field virus exposures.
Ključne riječi
foot-and-mouth disease (FMD) virus; tonsil; cattle; persistent infection; carrier state; nested-PCR
Hrčak ID:
24769
URI
Datum izdavanja:
20.8.2007.
Posjeta: 2.096 *