Sažetak sa skupa
Association between aryl hydrocarbon receptor and 4-hydroxynonenal in oxidative stress-mediated chronic rhinosinusitis with nasal polyps
Aigerim Kvarantan
; General hospital Ivo Pedišić Sisak, Department of otorhinolaryngology aqnd oral surgery, Sisak, Croatia
Vedran Balta
; Zagreb University, Faculty of science, Derpartment of biology, Zagreb, Croatia
Neven Žarković
; Ruđer Bošković research institute, Department of molecular medicine, Laboratory for oxidative stress
Tea Horvat
; Ruđer Bošković research institute, Department of molecular medicine, Laboratory for oxidative stress
Tea Vuković
; Ruđer Bošković research institute, Department of molecular medicine, Laboratory for oxidative stress
Kamelija Žarković
; University hospital Zagreb, Department of pathology, Zagreb, Croatia
Livije Kalogjera
; University hospital center Sestre milosrdnice, Department of ENT & head and neck surgery
Sažetak
Background: Сhronic rhinosinusitis with nasal polyps (CRSwNPs) is a distinct entity within the
chronic rhinosinusitis group of diseases, which are chronic upper airway diseases with several pheno- and
endotypes. Oxidative stress plays an important role in the pathogenesis of CRSwNPs.
Aim: The aim was to assess the association between the expression of the aryl hydrocarbon receptor
(AhR) and 4-hydroxynonenal (4-HNE) in patients with CRSwNPs.
Methods: The study included 26 patients who underwent endoscopic sinus surgery – 14 patients with
CRSwNPs, and 12 controls with healthy sinus mucosa. The expression of AhR and 4-HNE was assessed in
tissue samples using immunohistochemistry. The level of 4-HNE in serum samples was measured using the
ELISA assay. The total oxidative capacity (TOC) was assessed by measuring the peroxidase activity.
Results: Higher levels of 4-HNE expression were observed in tissues (3, range 1-3 vs. 0, range 0-0,
p<0.001) and serum (27.7±11.5 vs. 9.8±7.7 pmol/mg, p<0.001) samples of CRSwNPs patients, as compared
to healthy controls. A higher expression of AhR was found in inflammatory cells (plasma cells, lymphocytes,
eosinopholes) of CRSwNPs patients, compared to controls (3, range 1-3 vs. 2, range 1-2, p=0.001). There were
no differences in TOC across groups (0.0285±0.0207 vs. 0.02978±0.0197 µM H2O2 eq., p=0.848). Patients
with bronchial asthma (57%) had abundant eosinophiles in tissue samples. Patients with recalcitrant CRSwNPs
had higher 4-HNE serum levels, compared to non-recalcitrant cases (27.3 vs. 24.2 pmol/mg, p=0.339).
Conclusion: Patients suffering from CRSwNPs have oxidative stress mediated overexpression of AhR,
which is linked to a chronic inflammatory response in the paranasal sinus tissues.
Ključne riječi
chronic rhinosinusitis; nasal polyps; oxidative stress
Hrčak ID:
272883
URI
Datum izdavanja:
27.2.2022.
Posjeta: 321 *