Sažetak sa skupa
The interaction of IFN-γ, IL-4 and IL-5 with inflammation severity, predisposing factors and phenotype in chronic rhinosinusitis
Ana Penezić
; KBC Sestre milosrdnice, Klinika za otorinolaringologiju i kirurgiju glave i vrata
Tomislav Gregurić
; KBC Sestre milosrdnice, Klinički zavod za dijagnostičku i intervencijsku radiologiju
Anita Topić
; KBC Sestre milosrdnice, Klinički zavod za biokemiju
Marko Velimir Grgić
; KBC Sestre milosrdnice, Klinika za otorinolaringologiju i kirurgiju glave i vrata
Tomislav Baudoin
; KBC Sestre milosrdnice, Klinika za otorinolaringologiju i kirurgiju glave i vrata
Livije Kalogjera
; KBC Sestre milosrdnice, Klinika za otorinolaringologiju i kirurgiju glave i vrata
Sažetak
Introduction: Chronic rhinosinusitis (CRS) is a complex clinical syndrome, where the interaction of
different predisposing factors has an impact on symptoms and pattern of inflammation.
Aim: The aim of the study was to show the regulation of different cytokines (IFN-γ, IL-4 and IL-5)
related to CRS phenotypes and comorbidities.
Methods: The study included 50 patients with CRS according to EPOS criteria. Sinus and polyp
samples were collected at sinus surgery: 29 from non-allergic CRS without nasal polyps (CRSsNP), 9 from
allergic non-asthmatic CRSsNP, 17 from allergic asthma, 11 from non-allergic asthma, 5 from aspirin
intolerant CRS with nasal polyps (CRSwNP). The tissue homogenates were prepared immediately on ice and
incubated with protease inhibitor. Assays (R&D System, Quantikine ELISA, UK) for interleukins IL-4, IL-5
and for interferon gamma (IFN-γ) employed the quantitative sandwich enzyme immunoassay technique, CT
scans were scored with the Lund-Mackay scoring system. The Kruskal-Valis test was performed to compare
values of tissue homogenate concentrations of cytokines between groups.
Results: The study included 50 CRS (30 males) patients mean age of 43.2 years; 32 with CRSsNP and
18 with CRSwNP. IL-4 was not significantly different between the groups. IL-5 was significantly higher in
allergic asthma than in both CRSsNP and in non-allergic asthma than in CRSsNP with allergic sensitization.
Unexpectedly, IFN- γ was significantly higher in CRSsNP with allergic sensitization than in other groups. IL-
5 was the only cytokine that correlated with the CT score. IL-4 correlated significantly with both IL-5 and
IFN- γ.
Conclusion: The data confirmed significantly higher IL-5 concentrations in asthmatic patients which
are mainly CRSwNP, and in CRSwNP versus CRSsNP in general. IFN-γ concentrations in CRSsNP with
allergic sensitization are significantly higher than in any other subgroup. Up-regulation of IL-4 in ASA
intolerant subgroup suggested greater involvement of IL-4 in the pathogenesis of CRS in ASA intolerant
patients than in other subgroups.
Ključne riječi
chronic rhinosinusitis; interleukin 4; interleukin 5; interferon gamma
Hrčak ID:
272965
URI
Datum izdavanja:
27.2.2022.
Posjeta: 313 *