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https://doi.org/10.46419/vs.54.1.1

Semen quality in sheep

Yurani Arbodela Marin ; Semillero de Investigación y Grupo de Estudio en Caprinos y Ovinos (GECO-GaMMA), Facultad de Ciencias Agrarias, Universidad de Antioquia. Medellín, Colombia
Daniela Alejandra Gonzalez Gaviria ; Semillero de Investigación y Grupo de Estudio en Caprinos y Ovinos (GECO-GaMMA), Facultad de Ciencias Agrarias, Universidad de Antioquia. Medellín, Colombia
Leidy Katerine Lopera Mesa ; Semillero de Investigación y Grupo de Estudio en Caprinos y Ovinos (GECO-GaMMA), Facultad de Ciencias Agrarias, Universidad de Antioquia. Medellín, Colombia
Paula Andrea Lalinde Acevedo ; Grupo Reproducción, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia
Andrés Felipe Mejia ; Empresa de Biotecnología de la Reproducción Ge3, Medellín, Colombia
Walter D. Cardona Maya ; Grupo Reproducción, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia
Henry Cardona Cadavid ; Grupo de Investigación en Genética, Mejoramiento y Modelación Animal (GaMMA), Universidad de Antioquia, Medellín, Colombia

Sažetak

Reproductive evaluation, the selection of individual breeders, and reproductive biotechnologies are important tools in developing productive and reproductive rates. When choosing a male as a breeding future, determining its general health status and genotypic and phenotypic superiority are necessary. This study evaluated the conventional and functional seminal quality in ovine males. The semen of eleven ovines of different pure races was collected by electroejaculation. The following average values were observed for each conventional parameter: scrotal circumference (CE, 34.3 cm), seminal volume (vol, 1.63 mL), concentration (C, 768.4 x 106/mL), individual motility (MI, 80%), masal motility (MM, 4) and vigor (Vi, 3.7). On the other hand, functional analysis showed plasma membrane integrity (PMI) of 45.7%, mitochondrial membrane potential (MMP) of 38.5%, DNA fragmentation index (DFI) of 17.0%, lipoperoxidation of the membrane (LPO) of 32.7%, and the production of reactive oxygen species (ROS) of 28.56%. Additionally, a high and positive correlation was found for variables concerning seminal quality: Vi and MM, Vi and MI, MMP and MM, MMP and MI, PMI and MMP, as well as ROS and MMP. In contrast, two strongly negative correlations were obtained (C vs. Vol and LPO vs. CE). These are the first estimates of functional seminal quality evaluation of sheep in Colombia. In addition, this study provides a baseline for conventional and functional seminal parameters in the region.

Ključne riječi

semen quality; flow cytometry; reproduction; sheep; semen

Hrčak ID:

273742

URI

https://hrcak.srce.hr/273742

Datum izdavanja:

25.5.2022.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.327 *