Izvorni znanstveni članak
https://doi.org/10.3325/cmj.2021.62.21 5
Sex differences in oxidative stress level and antioxidative enzymes expression and activity in obese pre-diabetic elderly rats treated with metformin or liraglutide
Anita Matić
; Department of Physiology and Immunology, Faculty of Medicine, University Josip Juraj Strossmayer Osijek, Osijek, Croatia
Rosemary Vuković
; Department of Biology, University Josip Juraj Strossmayer Osijek, Osijek, Croatia
Marija Heffer
orcid.org/0000-0001-6770-7359
; Department of Medical Biology and Genetics, Faculty of Medicine Osijek, University Josip Juraj Strossmayer of Osijek, Osijek, Croatia
Marta Balog
; Department of Medical Biology and Genetics, Faculty of Medicine Osijek, University Josip Juraj Strossmayer of Osijek, Osijek, Croatia
Vedrana Ivić
; Department of Medical Biology and Genetics, Faculty of Medicine Osijek, University Josip Juraj Strossmayer of Osijek, Osijek, Croatia
Robert Gaspar
; Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary
Eszter Ducza
; Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Szeged, Hungary
Kalman F. Szucs
orcid.org/0000-0002-5878-4691
; Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Szeged, Hungary
Adrienn Seres
; Department of Pharmacology and Pharmacotherapy, Faculty of Medicine, University of Szeged, Szeged, Hungary
Sandor G. Vari
orcid.org/0000-0003-2962-2017
; International Research and Innovation in Medicine Program, Cedars-Sinai Medical Center, Los Angeles, CA, USA
Ines Drenjančević
orcid.org/0000-0003-4964-7721
; Department of Physiology and Immunology, Faculty of Medicine, University Josip Juraj Strossmayer Osijek, Osijek, Croatia
Sažetak
Aim To determine the effects of metformin or liraglutide on
oxidative stress level and antioxidative enzymes gene ex
-
pression and activity in the blood and vessels of pre-diabet
-
ic obese elderly Sprague-Dawley (SD) rats of both sexes.
Methods Male and female SD rats were assigned to the
following groups: a) control group (fed with standard ro
-
dent chow); b) high-fat and high-carbohydrate diet
(HSHFD) group fed with HSHFD from 20-65 weeks of age;
c) HSHFD+metformin treatment (50 mg/kg/d s.c.); and d)
HSHFD+liraglutide treatment (0.3 mg/kg/d s.c). Oxidative
stress parameters (ferric reducing ability of plasma and
thiobarbituric acid reactive substances) and superoxide dis
-
mutase (SOD), catalase (CAT), and glutathione peroxidase
(GPx) activity and gene expression were determined from
serum, aortas, and surface brain blood vessels (BBV).
Results HSHFD increased body weight in both sexes com
-
pared with the control group, while liraglutide prevented
this increase. Blood glucose level did not change. The lira
-
glutide group had a significantly increased antioxidative ca
-
pacity compared with the HSHFD group in both sexes. The
changes in antioxidative enzymes’ activities in plasma were
more pronounced in male groups. The changes in gene ex
-
pression of antioxidative enzymes were more prominent in
microvessels and may be attributed to weight gain preven
-
tion.
Conclusions Obesity and antidiabetic drugs caused sexrelated differences in the level of antioxidative parameters.
Liraglutide exhibited stronger antioxidative effects than
metformin. These results indicate that weight gain due to
HSHFD is crucial for developing oxidative stress and for in
-
hibiting antioxidative protective mechanisms
Ključne riječi
Hrčak ID:
278130
URI
Datum izdavanja:
24.6.2021.
Posjeta: 426 *