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Croatian Medical Journal, Vol. 62 No. 6, 2021.

Izvorni znanstveni članak

https://doi.org/10.3325/cmj.2021.62.590

Effects of gestational diabetes mellitus on the quality and quantity of blood hematopoietic stem cells: a case-control study

Maiza Tusimin ; Department of Obstetrics and Gynecology, Universiti Putra Malaysia, Serdang, Malaysia
Sara M. El. Ahmed ; Department of Pathology, Universiti Putra Malaysia, Serdang, Malaysia
Kai Wei Lee ; Department of Pre-Clinical Sciences, Universiti Tunku Abdul Rahman, Kampar, Malaysia
Ching Siew Mooi ; Department of Family Medicine, Universiti Putra Malaysia, Serdang, Malaysia
Sabariah Md Noor ; Department of Pathology, Universiti Putra Malaysia, Serdang, Malaysia
Norshariza Nordin ; Department of Biomedicine, Universiti Putra Malaysia, Serdang, Malaysia

Puni tekst: engleski pdf 229 Kb

str. 590-597

preuzimanja: 198

citiraj

Sažetak

Aim To evaluate the effects of gestational diabetes mellitus (GDM) on the quantity and quality of hematopoietic
stem cells (HSC).
Methods In this case-control study, HSC were isolated
from umbilical cord blood (UCB) procured at delivery from
63 mothers with GDM and 67 healthy mothers. Total nucleated cells (TNC) and CD34+ cells were quantified using BD
FACSCalibur flow cytometer. The quantity and quality of
stem cells were determined.
Results The GDM group had lower total cord blood volume and lower number of nucleated HSC compared with
healthy mothers. Regarding stem cell quantity parameters,
they had significantly lower UCB volume (P=0.041), TNC
count (P=0.022), total viable NC count (P=0.014), and
CD34+ percentage (P=0.014). Regarding the quality of
stem cells, they had significantly lower viable TNC percentage (P=0.015). The predictors for total TNC count were longer labor duration (adjusted B coefficient [p]: 0.031 [0.046]),
greater estimated blood loss (0.089 [0.005]), female neonates (12.322 [0.049]), and higher placenta weight (0.080
[0.033]). The predictors of total viable NC count were greater estimated blood loss (0.092 [0.003]), female neonates
(13.16 [0.035]), and greater placenta weight (0.083 [0.026]).
Conclusion The GDM group had much lower quantity
and quality of UCB stem cells. Our results should be taken
into consideration when drawing cord blood for unrelated
stem cell banking in an obstetric unit to ensure the obtaining of optimal cord blood samples and to avoid unnecessary expenses.

Ključne riječi

Hrčak ID:

278869

URI

https://hrcak.srce.hr/278869

Datum izdavanja:

23.12.2021.

Posjeta: 751 *