Izvorni znanstveni članak
Multiplex Mutagenically Separated Polymerase Chain Reaction Assay for Rapid Detection of Human Mitochondrial DNA Variations in Coding Area
Jin Wu
; Institut za forenzičku medicinu, Sichuan, Kina
Peng Ran
; Institut za forenzičku medicinu, Sichuan, Kina
Yingbi Li
; Institut za forenzičku medicinu, Sichuan, Kina
Jing Yan
; Institut za forenzičku medicinu, Sichuan, Kina
Miao Liao
; Institut za forenzičku medicinu, Sichuan, Kina
Yiping Hou
; Institut za forenzičku medicinu, Sichuan, Kina
Lin Zhang
; Institut za forenzičku medicinu, Sichuan, Kina
Sažetak
Recently, it has been recognized that information in the mitochondrial
DNA (mtDNA) coding region can provide additional forensic discrimination
with respect to the standard typing of the D-loop region, increasing
the forensic power of mtDNA testing, which is sometimes rather
limited. In the present study, we simultaneously typed ten single nucleotide
polymorphisms (SNP) in the coding region by use of mutagenically
separated polymerase chain reaction (MS-PCR) in the Chinese Chengdu
population. This technique, in which different-size allele-specific primers
were used, specifically amplified both alleles of mtDNA in the same
reaction. Subsequent gel electrophoresis showed ten of the allelic products
of different loci. Using multiplex MS-PCR, 30 primers were added
simultaneously into one reaction tube to identify ten SNPs. The mtDNA
variations of 160 individuals from the Chinese Chengdu population
were examined and classified into 18 haplotypes. The multiplex MS-PCR
method is suitable for large-scale screening studies of mtDNA variability
because it is both rapid and economical.
Ključne riječi
Mitochondrial DNA; Single nucleotide polymorphisms; mutagenically separated PCR; Chinese chengdu population
Hrčak ID:
26087
URI
Datum izdavanja:
15.2.2008.
Posjeta: 2.073 *