Veterinar, Vol. 60. No. 1., 2022.
Izvorni znanstveni članak
Mouse Myelinating Oligodendrocyte Differentiation In Vitro
Robert Rebac
; Moderna Switzerland GmbH. Basel, Švicarska
Snježana Kužir
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Ivan Alić
; Veterinarski fakultet Sveučilišta u Zagrebu, Zagreb, Hrvatska
Sažetak
Cells of the nervous system, neurons, and glia-cells originate from the same stem cell population with the expression of specific markers. The main goal of our study was to analyse the differentiation of oligodendrocytes and show the expression of cell stage-characteristic markers. The study was performed on cells isolated from newborn Thy1 mouse pups. Thy1 mice, under the promoter of the Thy1 gene, express a green fluorescent protein in some neurons. Oligodendrocyte progenitors were differentiated into mature, myelinating oligodendrocytes. The cells were fixed on the 1st, 3rd, 5th and 7th days of differentiation and analysed by immunocytochemistry. Our results showed that on the 1st day of differentiation, 99 % of cells were olig2, sox2, and nestin positive. Moreover, on the 1st day of differentiation 88 ± 2 % of cells were o4 and o1 positive, while only 10 % of cells were gfap positive. During cell differentiation, the proportion of olig2 positive cells decreased to 8 ± 2 %, while on the 5th day of differentiation we observed mature, myelinating oligodendrocytes, mbp, and plp positive cells. On the 7th day of differentiation, 13 ± 2 % of cells were mbp positive, while 84 ± 3 % of cells were plp positive. In conclusion, the results of our study showed that cells could be com pletely differentiated, from oligodendrocyte progenitors to mature, myelinating oligodendrocytes. Mature cells showed myelination of neighbor cells.
Ključne riječi
stem cells; differentiation; glia cells; immunocytochemistry; mouse
Hrčak ID:
284807
URI
Datum izdavanja:
20.9.2022.
Posjeta: 388 *