Izvorni znanstveni članak
Y-chromosomal Short Tandem Repeat Haplotypes in Southern Croatian Male Population Defined by 17 Loci
Jelena Ljubković
; Zavod za patologiju i forenzičku medicinu, Medicinski fakultet Sveučilišta u Splitu, Split, Hrvatska
Angela Stipišić
; Institut za javno zdravstvo, Split, Hrvatska
Davorka Sutlović
orcid.org/0000-0002-0102-7251
; Zavod za patologiju i forenzičku medicinu, Medicinski fakultet Sveučilišta u Splitu, Split, Hrvatska
Marija Definis-Gojanović
; Zavod za patologiju i forenzičku medicinu, Medicinski fakultet Sveučilišta u Splitu, Split, Hrvatska
Kajo Bučan
; Zavod za oftalmologiju, Klinički bolnički centar Split, Hrvatska
Šimun Anđelinović
; Zavod za patologiju i forenzičku medicinu, Medicinski fakultet Sveučilišta u Splitu, Split, Hrvatska
Sažetak
Aim To define the Y-chromosome genetic structure in a sample of
men from southern Croatia.
Methods Blood samples were collected from 166 unrelated healthy
men from southern Croatia at the Department of Forensic Medicine
and Biochemical Laboratory of University Hospital Split between
2004 and 2007. Genomic DNA was extracted using the standard
procedures. Seventeen Y-chromosome short tandem repeat (YSTR)
polymorphic loci (DYS456, DYS389I, DYS390, DYS389II,
DYS458, DYS19, DYS385, DYS393, DYS391, DYS439, DYS635,
DYS392, GATAH4, DYS437, DYS438, and DYS448) were analyzed
using AmpFlSTR Yfiler Polymerase Chain Reaction Amplification
Kit.
Results We observed 152 different haplotypes. Total haplotype
diversity was 0.997289 and 141 haplotypes (84.49%) were unique.
The most common haplotype was shared by only 4 men in the study
sample. The locus diversity ranged between 0.21292 for DYS392
and 0.75546 for DYS439 locus.
Conclusion The Y-chromosome structure in men from southern
Croatia is very diverse. Combination of Y chromosome 17 STR loci
may be used as a powerful tool for individual identification and parentage
analysis in the southern Croatian male population.
Ključne riječi
forensic science; Haplotype; Y-chromosomal STR; population data; Southern Croatia
Hrčak ID:
26153
URI
Datum izdavanja:
15.4.2008.
Posjeta: 2.497 *