Original scientific paper

https://doi.org/10.24099/vet.arhiv.1736

Association between single-nucleotide polymorphisms in ARID4A gene and sperm quality of Chinese water buffalo

Guanhong Lu ; College of Animal Science, Jilin University, Jilin, China; College of Life Sciences, Baicheng Normal University, Jilin, China
Chuanyu He ; TECON Biological Co., Ltd., Urumqi, China
Xi Yang ; College of Animal Science, Jilin University, Jilin, China
Yunyun Cheng ; College of Animal Science, Jilin University, Jilin, China
Rui Yang ; College of Animal Science, Jilin University, Jilin, China
Jie Song ; College of Animal Science, Jilin University, Jilin, China
Siyao Wang ; College of Animal Science, Jilin University, Jilin, China
Chunli Wang ; College of Animal Science, Jilin University, Jilin, China
Ze Ma ; College of Animal Science, Jilin University, Jilin, China
Jiapeng Li ; College of Animal Science, Jilin University, Jilin, China
Tianqi Feng ; College of Animal Science, Jilin University, Jilin, China
Linlin Hao ; College of Animal Science, Jilin University, Jilin, China
Changhong Li ; College of Life Sciences, Baicheng Normal University, Jilin, China

Abstract

ARID4A (AT-rich interaction domain 4A) is closely related to animal sperm quality traits. In the present study, the association between ARID4A gene polymorphisms of Chinese water buffalo (Bubalus bubalis) with sperm quality traits was examined, including ejaculate volume, sperm concentration, post-thaw sperm motility, and sperm abnormality of buffalo semen. Seven single-nucleotide polymorphisms (SNPs) of ARID4A gene were detected in 156 Chinese water buffaloes by Sanger sequencing and identifying overlap peaks. Among the SNPs, six were associated with at least one sperm quality trait. In brief, g.21192G>C, g.21285C>G, and g.21364A>G could be used as potential markers for selecting semen with low sperm abnormality, high ejaculate volume, sperm concentration, and sperm motility. Furthermore, 10 haplotypes (H1: -CTCGG, H2: GTGGCA, H3: GCGGCA, H4: GCTGCA, H5: GCTCGA, H6: GTGGGG, H7: GCTCCG, H8: -CGGGA, H9: GCGGCG, and H10: GTTGCA) were formed by the six SNPs through linkage disequilibrium analysis, and then 14 different combined haplotypes were collected. Correlation analysis showed that the combined H1H2 haplotype had the highest genotype frequency. Notably, the combined H1H2 haplotype had low sperm concentration, low sperm motility, and high sperm abnormality. The combined H2H3 haplotype could be used as a potential molecular marker for selecting semen with high sperm motility. In general, we illustrated a significant correlation between SNPs in ARID4A and sperm quality traits of Chinese water buffalo, which may be useful in the marker-assisted selection of buffalo breeding. This study was the first to analyze the genetic polymorphisms of ARID4A and association with sperm qualities of Chinese buffalo.

Keywords

Chinese water buffalo (Bubalus bubalis); ARID4A; sperm quality; single-nucleotide polymorphisms

Hrčak ID:

287648

URI

https://hrcak.srce.hr/287648

Publication date:

11.12.2022.

Article data in other languages: croatian

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