Izvorni znanstveni članak
https://doi.org/10.15255/CABEQ.2022.2119
Rheological and Low Field NMR Characterisation of Cystic Fibrosis Patient’s Sputum
G. Staltari
; Department of Engineering and Architecture, University of Trieste, Via Valerio 6/A, I-34127 Trieste, Italy
A. Biasin
; Department of Engineering and Architecture, University of Trieste, Via Valerio 6/A, I-34127 Trieste, Italy
L. Grassi
; Department of Engineering and Architecture, University of Trieste, Via Valerio 6/A, I-34127 Trieste, Italy
F. Gerin
; Department of Life Sciences, Cattinara University Hospital, Trieste University, Strada di Fiume 447, I-34149 Trieste, Italy
M. Maschio
; Institute for Maternal and Child Health, IRCCS Burlo Garofolo, Via Dell’Istria, 65, I-34137, Trieste, Italy
M. Confalonieri
; Cattinara University Hospital, Pulmonology Department, Strada di Fiume 447, I-34149, Trieste, Italy
G. Grassi
; Department of Life Sciences, Cattinara University Hospital, Trieste University, Strada di Fiume 447, I-34149 Trieste, Italy
M. Grassi
; Department of Engineering and Architecture, University of Trieste, Via Valerio 6/A, I-34127 Trieste, Italy
M. Abrami
; Department of Engineering and Architecture, University of Trieste, Via Valerio 6/A, I-34127 Trieste, Italy
Sažetak
The shear modulus G of the sputum obtained by expectoration from cystic fibrosis patients is fundamental to determine the mesh size of the polymeric network pervading the sputum, a parameter related to lung functionality. The Akaike criterion revealed that in 55.2 % of the examined samples, the best approach (among those considered) to determine G relied on the mechanical spectrum fitting by the generalised Maxwell model with relaxation times scaled by a factor 10. Thus, this approach was adopted to evaluate the mesh size distribution combining G knowledge with the determination of the average magnetic relaxation time (T2m) of sputum. As G and T2m determination can be negatively affected by sputum contamination by saliva, whose presence increases T2m and depresses G, we developed a proper “decontamination” procedure to obtain more reliable T2m and G estimations (necessary in 21 % of the samples). This procedure allowed to strengthen the T2m correlation with lung functionality evaluated by FEV1 (normalised air volume emitted in the first second during a spirometry test).
This work is licensed under a Creative Commons Attribution 4.0 International License.
Ključne riječi
rheology; low field NMR; cystic fibrosis; shear modulus; mesh size; saliva contamination
Hrčak ID:
293723
URI
Datum izdavanja:
14.2.2023.
Posjeta: 281 *