Izvorni znanstveni članak
Pathogenic Hantaviruses Elicit Different Immunoreactions in THP-1 Cells and Primary Monocytes and Induce Differentiation of Human Monocytes to Dendritic-Like Cells
Alemka Markotić
Lisa Hensley
Kathleen Daddario
Kristin Spik
Kevin Anderson
Connie Schmaljohn
Sažetak
Hantaviruses cause two important human illnesses, hemorrhagic fever with renal syndrome (HFRS) and hantavirus
pulmonary syndrome (HPS). Both syndromes are believed to be immune-mediated diseases. Monocytes/macrophages are
thought to be the main target cells for hantaviruses and important sources of and targets for cytokines/chemokines secretion.
THP-1 cells have been used extensively as models for primary monocytes in biocompatibility research. The aim of
our study was to determine if hantaviruses induce the same immunoreactions in THP-1 cells and primary monocytes/
macrophages and might therefore be suitable for immune studies of hantaviral infections. For that purpose we compared
various cytokines/chemokines and their receptors in THP-1 cell line and primary monocytes/macrophages. Infected primary
monocytes/macrophages induced mostly -chemokines and their receptors. In contrast, THP-1 cells, expressed receptors
for CXC chemokines. Surprisingly, infected macrophages underwent morphological changes toward dendriticlike
cells and increased expression of co-stimulatory molecules: CD40, CD80, CD83 and CD86. Our data indicate that
THP-1 cells are not ideal for in vitro research of the immunopathogenesis of hantaviruses in humans. Further, our studies
revealed potential roles for cytokines/chemokines in HFRS/HPS immunopathogenesis and point to intriguing possibilities
for the possible differentiation of infected macrophages to dendritic-like cells.
Ključne riječi
pathogenic hantaviruses; THP-1 cells; primary monocytes; dendritic-like cells; cytokines/chemokines
Hrčak ID:
26985
URI
Datum izdavanja:
3.12.2007.
Posjeta: 1.746 *