Veterinarska stanica, Vol. 54 No. 6, 2023.
Izvorni znanstveni članak
https://doi.org/10.46419/vs.54.6.9
Učinci ekstrakta Cytinus hypocistis (L.) L. u životinjskom modelu neoplazije inducirane papiloma virusom
Catarina Medeiros
; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
Ana Rita Silva
; Mountain Research Center (CIMO), Polytechnic Institute of Bragança (IPB), Campus de Santa Apolónia, Bragança, Portugal; Associate Laboratory for Sustainability and Technology in Mountains Regions (SusTEC), IPB, Campus de Santa Apolónia, Bragança, Portugal; Departament of Pharmaceutical Sciences, Facultad de Farmácia, CIETUS-IBSAL, Universidade de Salamanca, Salamanca, Spain
Tiago Ferreira
; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal
Lillian Barros
; Mountain Research Center (CIMO), Polytechnic Institute of Bragança (IPB), Campus de Santa Apolónia, Bragança, Portugal; Associate Laboratory for Sustainability and Technology in Mountains Regions (SusTEC), IPB, Campus de Santa Apolónia, Bragança, Portugal
Maria J. Neuparth
; Research Center in Physical Activity, Health and Leisure (CIAFEL), Faculty of Sports - University of Porto (FADEUP), Laboratory for Integrative and Translational Research in Population Health (ITR), Porto, Portugal; Toxicology Research Unit (TOXRUN), University Institute of Health Sciences, CESPU, Gandra, Portugal
Francisco Peixoto
; Chemistry Research Center (CQVR), UTAD, Vila Real, Portugal
Margarida M. S. M. Bastos
; Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE), Faculty of Engineering, University of Porto, Porto, Portugal; Associate Laboratory in Chemical Engineering (ALiCE), Faculty of Engineering, University of Porto, Porto, Portugal
Rui Medeiros
; Molecular Oncology and Viral Pathology Group, Research Center of IPO Porto (CI-IPOP)/RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto)/Porto Comprehensive Cancer Center (Porto.CCC), Porto, Portugal; Biomedicine Research Center (CEBIMED), Faculty of Health Sciences, Fernando Pessoa University, Porto, Portugal; Research Department, LPCC-Portuguese League Against Cancer (NRNorte), Estr. da Circunvalação, Porto, Portugal; Faculty of Medicine, University of Porto, Porto, Portugal; Institute of Biomedical Sciences Abel Salazar (ICBAS), Porto, Portugal
Rui M. Gil da Costa
orcid.org/0000-0002-2151-2449
; Molecular Oncology and Viral Pathology Group, Research Center of IPO Porto (CI-IPOP)/RISE@CI-IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto)/Porto Comprehensive Cancer Center (Porto.CCC), Porto, Portugal; Biomedicine Research Center (CEBIMED), Faculty of Health Sciences, Fernando Pessoa University, Porto, Portugal; Research Department, LPCC-Portuguese League Against Cancer (NRNorte), Estr. da Circunvalação, Porto, Portugal; Faculty of Medicine, University of Porto, Porto, Portugal; Institute of Biomedical Sciences Abel Salazar (ICBAS), Porto, Portugal; Post-graduate Programme in Adult Health (PPGSAD), Morphology Department and University Hospital (HUUFMA), Federal University of Maranhão (UFMA), São Luís, Brazil
Maria João Pires
; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal; Department of Veterinary Sciences, UTAD, Vila Real, Portugal
Ana I. Faustino-Rocha
orcid.org/0000-0001-5572-6317
; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal; Department of Zootechnics, School of Sciences and Technology, University of Évora, Évora, Portugal; Comprehensive Health Research Center, Évora, Portugal; Adelina GAMA, Department of Veterinary Sciences, UTAD, Vila Real, Portugal; Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal
Adelina Gama
; Department of Veterinary Sciences, UTAD, Vila Real, Portugal; Associate Laboratory for Animal and Veterinary Science (AL4AnimalS), Animal and Veterinary Research Centre (CECAV), UTAD, Vila Real, Portugal
Paula Alexandra Oliveira
orcid.org/0000-0001-9519-4044
; Centre for the Research and Technology of Agro-Environmental and Biological Sciences (CITAB), Inov4Agro, University of Trás-os-Montes and Alto Douro (UTAD), Vila Real, Portugal; Department of Veterinary Sciences, UTAD, Vila Real, Portugal
Sažetak
Infekcije određenim vrstama papiloma virusa, poput humanog papiloma virusa 16 (HPV16), povezane su s razvojem preneoplastičnih lezija i karcinoma anogenitalnog područja i područja glave i vrata. Ekstrakti Cytinus hypocistis (CH) sadrže tvari koje pokazuju antiproliferativna, antioksidativna, protuupalna i antibakterijska svojstva te bi stoga mogle predstavljati nove, obećavajuće terapijske spojeve. Cilj je ovog rada bio analizirati utjecaj topikalne primjene ekstrakta dobivenog iz C. hypocistis (L.) L. na K14-HPV16 i FVB/n miševima za procjenu njegovih terapijskih i toksikoloških svojstava. Za postizanje ciljeva istraživanja, trideset ženki miševa starosti 33-37 tjedana podijeljeno je u šest skupina (n=5/ skupini): I (HPV+CH3,1); II (HPV+CH6,2); III (HPV+CH12,4); IV (FVB/n+CH12,4); V (HPV+kontrola) i VI (FVB/n+kontrola). CH je tijekom 28 dana topikalno primijenjen na oba uha. Nakon tog razdoblja sve životinje su žrtvovane u svrhu prikupljanja rezulata. Lezije kože su histološki klasificirane. Toksikološki parametri uključivali su hematološke i biokemijske markere krvi te analizu oksidativnog stresa jetre. Transgenične životinje pokazale
su smanjenje srednje tjelesne mase, bez obzira na primijenjenu koncentraciju ekstrakta. Ekstrakt nije utjecao na fiziološke parametre, masu organa ili parametre biokemijskog i oksidativnog stresa. Histološki je dokazana prisutnost proliferativnih epitelnih lezija na koži i oralnoj sluznici K14-HPV16 miševa, bez povezanosti s primjenom ovog ekstrakta. Općenito, primjena CH ekstrakta nije utjecala na lezije kože te su ga životinje dobro podnosile u primijenjenim koncentracijama.
Ključne riječi
životinjski model; miš; prirodni spoj; toksikologija
Hrčak ID:
296405
URI
Datum izdavanja:
19.5.2023.
Posjeta: 368 *